Results 1 - 20 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13958 | 5' | -51 | NC_003521.1 | + | 135770 | 0.66 | 0.998683 |
Target: 5'- cGAAGGCGGUgGCGgcaGUggcGCUGCGg -3' miRNA: 3'- -CUUCUGCCGaUGCa--CAagaCGAUGCa -5' |
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13958 | 5' | -51 | NC_003521.1 | + | 148794 | 0.66 | 0.998417 |
Target: 5'- aGGAGccGCGGCUGCGgacGUUCaucgUGCgcgGCGa -3' miRNA: 3'- -CUUC--UGCCGAUGCa--CAAG----ACGa--UGCa -5' |
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13958 | 5' | -51 | NC_003521.1 | + | 105927 | 0.66 | 0.998417 |
Target: 5'- -uAGACGGuCUGCGUGUg--GCcgGCGc -3' miRNA: 3'- cuUCUGCC-GAUGCACAagaCGa-UGCa -5' |
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13958 | 5' | -51 | NC_003521.1 | + | 129908 | 0.66 | 0.998108 |
Target: 5'- cGGGGCGGCcGCGcgc-CUGCUGCGc -3' miRNA: 3'- cUUCUGCCGaUGCacaaGACGAUGCa -5' |
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13958 | 5' | -51 | NC_003521.1 | + | 142179 | 0.66 | 0.997749 |
Target: 5'- -cAGGCGGCUguaGUGcgUCUGCUcGCGc -3' miRNA: 3'- cuUCUGCCGAug-CACa-AGACGA-UGCa -5' |
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13958 | 5' | -51 | NC_003521.1 | + | 31481 | 0.66 | 0.997749 |
Target: 5'- -uGGACGGCUuCGUGgagaguUUCUGCgacuuucucgACGUg -3' miRNA: 3'- cuUCUGCCGAuGCAC------AAGACGa---------UGCA- -5' |
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13958 | 5' | -51 | NC_003521.1 | + | 44083 | 0.66 | 0.997749 |
Target: 5'- cGgcGGCGGCUGacccggCUGCUGCGa -3' miRNA: 3'- -CuuCUGCCGAUgcacaaGACGAUGCa -5' |
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13958 | 5' | -51 | NC_003521.1 | + | 123077 | 0.66 | 0.997335 |
Target: 5'- ---aGCGGCgGCGUGUcgCUGCcGCGg -3' miRNA: 3'- cuucUGCCGaUGCACAa-GACGaUGCa -5' |
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13958 | 5' | -51 | NC_003521.1 | + | 69568 | 0.67 | 0.996317 |
Target: 5'- cGAGGACGGCgccgcccUGUG-UCgggGCUGCGc -3' miRNA: 3'- -CUUCUGCCGau-----GCACaAGa--CGAUGCa -5' |
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13958 | 5' | -51 | NC_003521.1 | + | 129944 | 0.67 | 0.995698 |
Target: 5'- cGAGACGGCgca--GUUCUGCcGCGg -3' miRNA: 3'- cUUCUGCCGaugcaCAAGACGaUGCa -5' |
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13958 | 5' | -51 | NC_003521.1 | + | 201758 | 0.67 | 0.995632 |
Target: 5'- -cGGGCGGCUagggucaGCGUGUUgcGCUGCa- -3' miRNA: 3'- cuUCUGCCGA-------UGCACAAgaCGAUGca -5' |
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13958 | 5' | -51 | NC_003521.1 | + | 239557 | 0.67 | 0.995632 |
Target: 5'- -cGGGCGGCUagggucaGCGUGUUgcGCUGCa- -3' miRNA: 3'- cuUCUGCCGA-------UGCACAAgaCGAUGca -5' |
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13958 | 5' | -51 | NC_003521.1 | + | 93314 | 0.67 | 0.995497 |
Target: 5'- aGGGACGGgaGCGggGUUCUuuuuugagggugcgGCUACGg -3' miRNA: 3'- cUUCUGCCgaUGCa-CAAGA--------------CGAUGCa -5' |
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13958 | 5' | -51 | NC_003521.1 | + | 132635 | 0.67 | 0.994207 |
Target: 5'- cGgcGGCGGCgccaccgGCG-GUUCUGCUuccuCGUc -3' miRNA: 3'- -CuuCUGCCGa------UGCaCAAGACGAu---GCA- -5' |
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13958 | 5' | -51 | NC_003521.1 | + | 120906 | 0.67 | 0.994207 |
Target: 5'- cGggGGCGGCUGCuugcGUUUgcgGCUGCc- -3' miRNA: 3'- -CuuCUGCCGAUGca--CAAGa--CGAUGca -5' |
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13958 | 5' | -51 | NC_003521.1 | + | 191684 | 0.68 | 0.993319 |
Target: 5'- cGAGGACGGCgaggcgucUGCGUGUUaaacgGCUguacGCGg -3' miRNA: 3'- -CUUCUGCCG--------AUGCACAAga---CGA----UGCa -5' |
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13958 | 5' | -51 | NC_003521.1 | + | 213841 | 0.68 | 0.991217 |
Target: 5'- --cGGCuGCUACGUGgcgCUGCUGuCGg -3' miRNA: 3'- cuuCUGcCGAUGCACaa-GACGAU-GCa -5' |
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13958 | 5' | -51 | NC_003521.1 | + | 150660 | 0.68 | 0.991217 |
Target: 5'- cGAAGGCGGCUcuccggcgGCGgcgccgCUGCUGCu- -3' miRNA: 3'- -CUUCUGCCGA--------UGCacaa--GACGAUGca -5' |
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13958 | 5' | -51 | NC_003521.1 | + | 83884 | 0.68 | 0.988624 |
Target: 5'- gGAGGugGGCUACGgcaaaGUcaUCcGCUGCa- -3' miRNA: 3'- -CUUCugCCGAUGCa----CA--AGaCGAUGca -5' |
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13958 | 5' | -51 | NC_003521.1 | + | 229317 | 0.68 | 0.988624 |
Target: 5'- uGggGACGGCU-Ca---UCUGCUGCGa -3' miRNA: 3'- -CuuCUGCCGAuGcacaAGACGAUGCa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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