Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13964 | 5' | -50.4 | NC_003521.1 | + | 59565 | 0.66 | 0.999024 |
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Target: 5'- cGGCGCCGCGg------GGGUUUCGUc -3' miRNA: 3'- -CCGCGGCGCaacaauaUUCGGAGUAc -5' |
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| 13964 | 5' | -50.4 | NC_003521.1 | + | 149766 | 0.66 | 0.999024 |
|
Target: 5'- gGGCgGCCGCGcg---GUAcGCCUCAa- -3' miRNA: 3'- -CCG-CGGCGCaacaaUAUuCGGAGUac -5' |
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| 13964 | 5' | -50.4 | NC_003521.1 | + | 192061 | 0.66 | 0.999024 |
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Target: 5'- cGGCGuCCGCGgcgGUUugugAGGCgUCGg- -3' miRNA: 3'- -CCGC-GGCGCaa-CAAua--UUCGgAGUac -5' |
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| 13964 | 5' | -50.4 | NC_003521.1 | + | 219166 | 0.66 | 0.999149 |
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Target: 5'- aGGCGCCGCagcgUGUUccaGUGgcggcugagccccaGGCC-CAUGa -3' miRNA: 3'- -CCGCGGCGca--ACAA---UAU--------------UCGGaGUAC- -5' |
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| 13964 | 5' | -50.4 | NC_003521.1 | + | 25570 | 0.66 | 0.999199 |
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Target: 5'- aGGCgGCCGCGggGacagGUGGGCUuguUCAUc -3' miRNA: 3'- -CCG-CGGCGCaaCaa--UAUUCGG---AGUAc -5' |
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| 13964 | 5' | -50.4 | NC_003521.1 | + | 123351 | 0.66 | 0.999199 |
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Target: 5'- aGCGCCGCGgccGU----GGCCUCc-- -3' miRNA: 3'- cCGCGGCGCaa-CAauauUCGGAGuac -5' |
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| 13964 | 5' | -50.4 | NC_003521.1 | + | 59175 | 0.66 | 0.999199 |
|
Target: 5'- cGGCGCCGCGggcagucaggagacGUUAaauauccUGAGUCgCGUGg -3' miRNA: 3'- -CCGCGGCGCaa------------CAAU-------AUUCGGaGUAC- -5' |
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| 13964 | 5' | -50.4 | NC_003521.1 | + | 111951 | 0.66 | 0.999199 |
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Target: 5'- uGGCGCgGCGgcaggAUGAGUCUguUGc -3' miRNA: 3'- -CCGCGgCGCaacaaUAUUCGGAguAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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