Results 41 - 48 of 48 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13964 | 5' | -50.4 | NC_003521.1 | + | 192061 | 0.66 | 0.999024 |
Target: 5'- cGGCGuCCGCGgcgGUUugugAGGCgUCGg- -3' miRNA: 3'- -CCGC-GGCGCaa-CAAua--UUCGgAGUac -5' |
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13964 | 5' | -50.4 | NC_003521.1 | + | 192238 | 0.69 | 0.990667 |
Target: 5'- cGGCGCCGCGggaggGUggccguuAGCCgUCAc- -3' miRNA: 3'- -CCGCGGCGCaa---CAauau---UCGG-AGUac -5' |
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13964 | 5' | -50.4 | NC_003521.1 | + | 203408 | 0.68 | 0.99288 |
Target: 5'- cGGCGCCGCGccacacgGUcAGGCC-CGUGu -3' miRNA: 3'- -CCGCGGCGCaacaa--UA-UUCGGaGUAC- -5' |
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13964 | 5' | -50.4 | NC_003521.1 | + | 210130 | 1.12 | 0.009515 |
Target: 5'- cGGCGCCGCGUUGUUAUAAGCCUCAUGa -3' miRNA: 3'- -CCGCGGCGCAACAAUAUUCGGAGUAC- -5' |
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13964 | 5' | -50.4 | NC_003521.1 | + | 211883 | 0.66 | 0.999346 |
Target: 5'- gGGCGCCGCGcgUGgUAUAGugcGCCa---- -3' miRNA: 3'- -CCGCGGCGCa-ACaAUAUU---CGGaguac -5' |
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13964 | 5' | -50.4 | NC_003521.1 | + | 213801 | 0.7 | 0.980705 |
Target: 5'- uGCGCCGCGccug---GAGCCUCGg- -3' miRNA: 3'- cCGCGGCGCaacaauaUUCGGAGUac -5' |
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13964 | 5' | -50.4 | NC_003521.1 | + | 219166 | 0.66 | 0.999149 |
Target: 5'- aGGCGCCGCagcgUGUUccaGUGgcggcugagccccaGGCC-CAUGa -3' miRNA: 3'- -CCGCGGCGca--ACAA---UAU--------------UCGGaGUAC- -5' |
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13964 | 5' | -50.4 | NC_003521.1 | + | 222121 | 0.66 | 0.999024 |
Target: 5'- aGGCGCUGCGccgGgaagaAUGAGCuCUgAUGa -3' miRNA: 3'- -CCGCGGCGCaa-Caa---UAUUCG-GAgUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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