Results 21 - 40 of 68 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13976 | 5' | -61.1 | NC_003521.1 | + | 131872 | 0.67 | 0.734096 |
Target: 5'- aCGGCCaucgUGCcGCGCGUGUcgGgCAGCg-- -3' miRNA: 3'- -GCCGG----ACGaCGCGCACA--CgGUCGagu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 130072 | 0.67 | 0.723803 |
Target: 5'- gCGGCCUGCUcuCGCuggccgaGUGgaacUGCCAGCUg- -3' miRNA: 3'- -GCCGGACGAc-GCG-------CAC----ACGGUCGAgu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 22415 | 0.67 | 0.715317 |
Target: 5'- uGGCCaUGCUGCGCGgcgugGCCgAGUa-- -3' miRNA: 3'- gCCGG-ACGACGCGCaca--CGG-UCGagu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 110821 | 0.67 | 0.715317 |
Target: 5'- gCGGCC-GUUGCGCGccgccGCCGGCgCAg -3' miRNA: 3'- -GCCGGaCGACGCGCaca--CGGUCGaGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 110200 | 0.67 | 0.715317 |
Target: 5'- aGGCCgcugGCgUGCGCGUGUuuguacuuGCCguAGCUg- -3' miRNA: 3'- gCCGGa---CG-ACGCGCACA--------CGG--UCGAgu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 183913 | 0.67 | 0.714371 |
Target: 5'- aGGCggUGCUGCgGCGcUGUcgcgacgGCCAGCUCu -3' miRNA: 3'- gCCGg-ACGACG-CGC-ACA-------CGGUCGAGu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 91699 | 0.67 | 0.705827 |
Target: 5'- gGGCgUGCUGCG-GUcugcGUGCC-GCUCc -3' miRNA: 3'- gCCGgACGACGCgCA----CACGGuCGAGu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 73968 | 0.67 | 0.696282 |
Target: 5'- uGuGCCUGCUGUGCGagcucaUGU-CCuGCUCGu -3' miRNA: 3'- gC-CGGACGACGCGC------ACAcGGuCGAGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 163647 | 0.67 | 0.686687 |
Target: 5'- gGGCCUcGgUGCGCGUcucgGUGCUgcggagcuGCUCAa -3' miRNA: 3'- gCCGGA-CgACGCGCA----CACGGu-------CGAGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 136773 | 0.68 | 0.677052 |
Target: 5'- uGGCCUacCUGCGCGacaGCgGGCUCAc -3' miRNA: 3'- gCCGGAc-GACGCGCacaCGgUCGAGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 168833 | 0.68 | 0.677052 |
Target: 5'- gGGCCccagcgaGCUGCGCGUcacGgacGCCgGGCUCAu -3' miRNA: 3'- gCCGGa------CGACGCGCA---Ca--CGG-UCGAGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 53194 | 0.68 | 0.667384 |
Target: 5'- uGGCCaGCgGCGCGcG-GcCCGGCUCGc -3' miRNA: 3'- gCCGGaCGaCGCGCaCaC-GGUCGAGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 13981 | 0.68 | 0.667384 |
Target: 5'- gGGCCUGCccgGCGCGgcgGCgGGCg-- -3' miRNA: 3'- gCCGGACGa--CGCGCacaCGgUCGagu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 22356 | 0.68 | 0.65769 |
Target: 5'- gGGCCacUGCgcccacgGCGUG-GUGCCGGC-CAu -3' miRNA: 3'- gCCGG--ACGa------CGCGCaCACGGUCGaGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 73167 | 0.68 | 0.65769 |
Target: 5'- uCGGCCUGCgaccugGaCGCGgagcugGCCGGCaUCGa -3' miRNA: 3'- -GCCGGACGa-----C-GCGCaca---CGGUCG-AGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 198791 | 0.68 | 0.647977 |
Target: 5'- gGGUagcaGCUGCGUG-GUGCCGGCg-- -3' miRNA: 3'- gCCGga--CGACGCGCaCACGGUCGagu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 193265 | 0.68 | 0.647977 |
Target: 5'- aCGGUCgGCUGCggaGCGgGUGCCGGgaCGg -3' miRNA: 3'- -GCCGGaCGACG---CGCaCACGGUCgaGU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 33935 | 0.68 | 0.638253 |
Target: 5'- uGGUgCUGCUGUGCGgcGUGCgCGGCgagCAg -3' miRNA: 3'- gCCG-GACGACGCGCa-CACG-GUCGa--GU- -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 98820 | 0.68 | 0.63728 |
Target: 5'- aGGCguugcagCUGCUugGCGCGg--GCCAGCUCc -3' miRNA: 3'- gCCG-------GACGA--CGCGCacaCGGUCGAGu -5' |
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13976 | 5' | -61.1 | NC_003521.1 | + | 232918 | 0.69 | 0.618798 |
Target: 5'- uGGaCCUGCUG-GUGUGUGUCuuucuacuAGCUCu -3' miRNA: 3'- gCC-GGACGACgCGCACACGG--------UCGAGu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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