Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14059 | 3' | -60.1 | NC_003521.1 | + | 195646 | 0.67 | 0.805836 |
Target: 5'- uCCgagGCCAGGGagucGcaccaccacuuacGCGUGCgguCCCAGUAGGu -3' miRNA: 3'- -GGa--CGGUCCC----C-------------CGCAUG---GGGUCAUCC- -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 202739 | 0.67 | 0.789856 |
Target: 5'- --cGgCAGGGGcGCGgggACCCCGuGUGGa -3' miRNA: 3'- ggaCgGUCCCC-CGCa--UGGGGU-CAUCc -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 218548 | 0.67 | 0.806664 |
Target: 5'- gCUGCCAGGcGaGGCGUgguagACCCCGa---- -3' miRNA: 3'- gGACGGUCC-C-CCGCA-----UGGGGUcaucc -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 219422 | 0.71 | 0.594674 |
Target: 5'- gCUGCCAGaGGGcCGUAUCCCAGc--- -3' miRNA: 3'- gGACGGUCcCCC-GCAUGGGGUCaucc -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 233443 | 0.66 | 0.867843 |
Target: 5'- --gGCCGGGcaGGGCGUAacguaCCCAGgagacGGa -3' miRNA: 3'- ggaCGGUCC--CCCGCAUg----GGGUCau---CC- -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 235709 | 0.68 | 0.772551 |
Target: 5'- ----aCAGGGGGCGUaGCCCgAGUAa- -3' miRNA: 3'- ggacgGUCCCCCGCA-UGGGgUCAUcc -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 239387 | 0.66 | 0.867843 |
Target: 5'- gUUGCUAGGcGGCGUuCCCUGGUGu- -3' miRNA: 3'- gGACGGUCCcCCGCAuGGGGUCAUcc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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