Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 14059 | 3' | -60.1 | NC_003521.1 | + | 36305 | 0.7 | 0.652056 |
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Target: 5'- --gGCCGGguGGGGCGcACCCuCAGUcGGGg -3' miRNA: 3'- ggaCGGUC--CCCCGCaUGGG-GUCA-UCC- -5' |
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| 14059 | 3' | -60.1 | NC_003521.1 | + | 68032 | 0.7 | 0.652056 |
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Target: 5'- aCC-GCCAcGGGcGGCGUGguccgcggcgcCCCCGGUGGc -3' miRNA: 3'- -GGaCGGU-CCC-CCGCAU-----------GGGGUCAUCc -5' |
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| 14059 | 3' | -60.1 | NC_003521.1 | + | 219422 | 0.71 | 0.594674 |
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Target: 5'- gCUGCCAGaGGGcCGUAUCCCAGc--- -3' miRNA: 3'- gGACGGUCcCCC-GCAUGGGGUCaucc -5' |
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| 14059 | 3' | -60.1 | NC_003521.1 | + | 117496 | 0.71 | 0.575675 |
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Target: 5'- gCCUGuCCGGcGGGGCGUacggcgguaugcACUCC-GUGGGc -3' miRNA: 3'- -GGAC-GGUC-CCCCGCA------------UGGGGuCAUCC- -5' |
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| 14059 | 3' | -60.1 | NC_003521.1 | + | 92080 | 0.73 | 0.483557 |
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Target: 5'- aCCggggGUCGGGGGGUgcgcagggcgGUGCCCCGGgucGGa -3' miRNA: 3'- -GGa---CGGUCCCCCG----------CAUGGGGUCau-CC- -5' |
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| 14059 | 3' | -60.1 | NC_003521.1 | + | 90288 | 0.75 | 0.36058 |
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Target: 5'- --cGCCcccguAGGGGGgGUACCCCGGUAa- -3' miRNA: 3'- ggaCGG-----UCCCCCgCAUGGGGUCAUcc -5' |
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| 14059 | 3' | -60.1 | NC_003521.1 | + | 150492 | 1.12 | 0.001419 |
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Target: 5'- cCCUGCCAGGGGGCGUACCCCAGUAGGg -3' miRNA: 3'- -GGACGGUCCCCCGCAUGGGGUCAUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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