Results 21 - 27 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
14059 | 3' | -60.1 | NC_003521.1 | + | 239387 | 0.66 | 0.867843 |
Target: 5'- gUUGCUAGGcGGCGUuCCCUGGUGu- -3' miRNA: 3'- gGACGGUCCcCCGCAuGGGGUCAUcc -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 135991 | 0.66 | 0.853555 |
Target: 5'- gCgUGUCGGGGGGCcgcGUcacGCCCguGUucucGGGg -3' miRNA: 3'- -GgACGGUCCCCCG---CA---UGGGguCA----UCC- -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 23229 | 0.66 | 0.838565 |
Target: 5'- cCCUGCUGGuccGGGGCGgACCCgGGg--- -3' miRNA: 3'- -GGACGGUC---CCCCGCaUGGGgUCaucc -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 27506 | 0.66 | 0.853555 |
Target: 5'- aCUcCaCGGGGGGCGUgcggcccgaggcGCCCaCGGUGGc -3' miRNA: 3'- gGAcG-GUCCCCCGCA------------UGGG-GUCAUCc -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 124190 | 0.66 | 0.838565 |
Target: 5'- gCCUGCCGgcuGGGGGUGagcggGCCCUuucuGGc -3' miRNA: 3'- -GGACGGU---CCCCCGCa----UGGGGucauCC- -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 10497 | 0.66 | 0.860789 |
Target: 5'- --cGCCggcGGGGauGGCGUGgccCCCCAGgcGGc -3' miRNA: 3'- ggaCGG---UCCC--CCGCAU---GGGGUCauCC- -5' |
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14059 | 3' | -60.1 | NC_003521.1 | + | 233443 | 0.66 | 0.867843 |
Target: 5'- --gGCCGGGcaGGGCGUAacguaCCCAGgagacGGa -3' miRNA: 3'- ggaCGGUCC--CCCGCAUg----GGGUCau---CC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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