Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14059 | 3' | -60.1 | NC_003521.1 | + | 135991 | 0.66 | 0.853555 |
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Target: 5'- gCgUGUCGGGGGGCcgcGUcacGCCCguGUucucGGGg -3' miRNA: 3'- -GgACGGUCCCCCG---CA---UGGGguCA----UCC- -5' |
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| 14059 | 3' | -60.1 | NC_003521.1 | + | 23229 | 0.66 | 0.838565 |
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Target: 5'- cCCUGCUGGuccGGGGCGgACCCgGGg--- -3' miRNA: 3'- -GGACGGUC---CCCCGCaUGGGgUCaucc -5' |
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| 14059 | 3' | -60.1 | NC_003521.1 | + | 34279 | 0.68 | 0.74579 |
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Target: 5'- uCCUGUCAcGGGGGCGgGCCaCGGgcGu -3' miRNA: 3'- -GGACGGU-CCCCCGCaUGGgGUCauCc -5' |
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| 14059 | 3' | -60.1 | NC_003521.1 | + | 5313 | 0.69 | 0.718243 |
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Target: 5'- --cGCgGGGGcGGCGUGCCCCGcc-GGc -3' miRNA: 3'- ggaCGgUCCC-CCGCAUGGGGUcauCC- -5' |
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| 14059 | 3' | -60.1 | NC_003521.1 | + | 68032 | 0.7 | 0.652056 |
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Target: 5'- aCC-GCCAcGGGcGGCGUGguccgcggcgcCCCCGGUGGc -3' miRNA: 3'- -GGaCGGU-CCC-CCGCAU-----------GGGGUCAUCc -5' |
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| 14059 | 3' | -60.1 | NC_003521.1 | + | 36305 | 0.7 | 0.652056 |
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Target: 5'- --gGCCGGguGGGGCGcACCCuCAGUcGGGg -3' miRNA: 3'- ggaCGGUC--CCCCGCaUGGG-GUCA-UCC- -5' |
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| 14059 | 3' | -60.1 | NC_003521.1 | + | 150492 | 1.12 | 0.001419 |
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Target: 5'- cCCUGCCAGGGGGCGUACCCCAGUAGGg -3' miRNA: 3'- -GGACGGUCCCCCGCAUGGGGUCAUCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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