Results 21 - 25 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1435 | 3' | -57.4 | NC_001335.1 | + | 11823 | 0.66 | 0.612166 |
Target: 5'- cCGGACG-GCACCgGCUccaACgCCGACc -3' miRNA: 3'- aGCUUGCuCGUGGgCGAc--UG-GGCUGu -5' |
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1435 | 3' | -57.4 | NC_001335.1 | + | 5691 | 0.66 | 0.612166 |
Target: 5'- gUCGAGCaGGUGCUCGC-GGCCaGGCAg -3' miRNA: 3'- -AGCUUGcUCGUGGGCGaCUGGgCUGU- -5' |
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1435 | 3' | -57.4 | NC_001335.1 | + | 18633 | 0.66 | 0.612166 |
Target: 5'- cCGGACuccGGUACCCGCU-ACCUGAa- -3' miRNA: 3'- aGCUUGc--UCGUGGGCGAcUGGGCUgu -5' |
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1435 | 3' | -57.4 | NC_001335.1 | + | 16622 | 0.66 | 0.612166 |
Target: 5'- cCGGuaGCGAGCACagggCCGa-GGCCCGugAa -3' miRNA: 3'- aGCU--UGCUCGUG----GGCgaCUGGGCugU- -5' |
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1435 | 3' | -57.4 | NC_001335.1 | + | 4968 | 0.66 | 0.622999 |
Target: 5'- gCGGuguGCGAGUagACCCcgccgccuccguGCUGgaGCCCGACGa -3' miRNA: 3'- aGCU---UGCUCG--UGGG------------CGAC--UGGGCUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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