Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14464 | 5' | -56.3 | NC_003521.1 | + | 28936 | 0.67 | 0.896451 |
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Target: 5'- gAUCACCUguguagcgcGGUCCGGggGGAGGAUg- -3' miRNA: 3'- aUGGUGGA---------CCGGGUCuaCCUCUUGaa -5' |
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| 14464 | 5' | -56.3 | NC_003521.1 | + | 16890 | 0.7 | 0.757707 |
|
Target: 5'- gGCCAUCUGGaggAGGUGGAGGACg- -3' miRNA: 3'- aUGGUGGACCgggUCUACCUCUUGaa -5' |
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| 14464 | 5' | -56.3 | NC_003521.1 | + | 16813 | 0.7 | 0.757707 |
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Target: 5'- gACCGCCgGGCCCgccgcugccgcaGGA-GGAGGACa- -3' miRNA: 3'- aUGGUGGaCCGGG------------UCUaCCUCUUGaa -5' |
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| 14464 | 5' | -56.3 | NC_003521.1 | + | 8625 | 0.69 | 0.803613 |
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Target: 5'- cACCACCcgcgggGGCCCAuGGcgugGGAGGACg- -3' miRNA: 3'- aUGGUGGa-----CCGGGU-CUa---CCUCUUGaa -5' |
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| 14464 | 5' | -56.3 | NC_003521.1 | + | 847 | 0.68 | 0.861246 |
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Target: 5'- -uCCugCUGGCgCAGAUccccguggaacGGAGAACg- -3' miRNA: 3'- auGGugGACCGgGUCUA-----------CCUCUUGaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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