miRNA: 3'- aUGGUGGaCCGGGUCUACCu--CUUgaa -5'
miRNA: 3'- aUG-GUGGa--CCGGGuCUACC-UCUUGAA- -5'
miRNA: 3'- aUG-GUGgACCGGGUC-----UACCUCUUGaa -5'
miRNA: 3'- -AUGgUGGaCCGGGUCuaCCUC--UUGaa -5'
miRNA: 3'- -AUGGUGGACCGGGUCUACCUCUUGAA- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14464 | 5' | -56.3 | NC_003521.1 | + | 84878 | 0.66 | 0.908971 |
|
Target: 5'- gGCCGCCcGGUCCGGGUGGcgcGAGu-- -3' miRNA: 3'- aUGGUGGaCCGGGUCUACCu--CUUgaa -5' |
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| 14464 | 5' | -56.3 | NC_003521.1 | + | 168115 | 0.67 | 0.902826 |
|
Target: 5'- cACgCGCCcggGGUCCuGGUGGaAGAACUUg -3' miRNA: 3'- aUG-GUGGa--CCGGGuCUACC-UCUUGAA- -5' |
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| 14464 | 5' | -56.3 | NC_003521.1 | + | 36772 | 0.67 | 0.896451 |
|
Target: 5'- gACgCGCgUGGCCUGGcgggcGUGGAGGACg- -3' miRNA: 3'- aUG-GUGgACCGGGUC-----UACCUCUUGaa -5' |
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| 14464 | 5' | -56.3 | NC_003521.1 | + | 239835 | 0.67 | 0.889849 |
|
Target: 5'- gUACgGCC-GGCCCAGcgGGAGcaGACc- -3' miRNA: 3'- -AUGgUGGaCCGGGUCuaCCUC--UUGaa -5' |
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| 14464 | 5' | -56.3 | NC_003521.1 | + | 112655 | 1.03 | 0.008268 |
|
Target: 5'- cUACCACCUGGCCCAGAUGGAGAACUUc -3' miRNA: 3'- -AUGGUGGACCGGGUCUACCUCUUGAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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