Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14610 | 5' | -54.3 | NC_003521.1 | + | 83713 | 0.7 | 0.895761 |
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Target: 5'- gGCGUCCaagGGGUccagGGC-CUCGUGCUCc -3' miRNA: 3'- -CGCGGGa--CCUAca--UCGaGAGCAUGAG- -5' |
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| 14610 | 5' | -54.3 | NC_003521.1 | + | 145437 | 0.7 | 0.889188 |
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Target: 5'- gGCGCCUcggUGGAccUGU-GCUUcggCGUGCUCa -3' miRNA: 3'- -CGCGGG---ACCU--ACAuCGAGa--GCAUGAG- -5' |
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| 14610 | 5' | -54.3 | NC_003521.1 | + | 156637 | 0.7 | 0.875387 |
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Target: 5'- aCGCCCUGGGcuuc-CUCuUCGUGCUCg -3' miRNA: 3'- cGCGGGACCUacaucGAG-AGCAUGAG- -5' |
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| 14610 | 5' | -54.3 | NC_003521.1 | + | 165311 | 0.7 | 0.868167 |
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Target: 5'- -aGCuCCUGGAUG-AGCUcCUUGUagcGCUCg -3' miRNA: 3'- cgCG-GGACCUACaUCGA-GAGCA---UGAG- -5' |
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| 14610 | 5' | -54.3 | NC_003521.1 | + | 39603 | 0.71 | 0.829097 |
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Target: 5'- cUGCCCUGGGUGgcGGCgUCUCGcaaUGCUUu -3' miRNA: 3'- cGCGGGACCUACa-UCG-AGAGC---AUGAG- -5' |
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| 14610 | 5' | -54.3 | NC_003521.1 | + | 97411 | 0.71 | 0.825772 |
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Target: 5'- -aGCCCUGGcccaggcgaaAGCUCUCGUcGCUCa -3' miRNA: 3'- cgCGGGACCuaca------UCGAGAGCA-UGAG- -5' |
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| 14610 | 5' | -54.3 | NC_003521.1 | + | 53917 | 0.72 | 0.815635 |
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Target: 5'- gGCGCCCcccuUGGAcgucagGUAGCUggugaucuugugcugCUCGUACUUg -3' miRNA: 3'- -CGCGGG----ACCUa-----CAUCGA---------------GAGCAUGAG- -5' |
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| 14610 | 5' | -54.3 | NC_003521.1 | + | 121133 | 0.73 | 0.738945 |
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Target: 5'- aCGCCgUGGAgcu-GCUgUCGUGCUCg -3' miRNA: 3'- cGCGGgACCUacauCGAgAGCAUGAG- -5' |
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| 14610 | 5' | -54.3 | NC_003521.1 | + | 67930 | 0.75 | 0.659903 |
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Target: 5'- cCGCCCUGGAcaUGgcccugGGCUCUUG-GCUCu -3' miRNA: 3'- cGCGGGACCU--ACa-----UCGAGAGCaUGAG- -5' |
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| 14610 | 5' | -54.3 | NC_003521.1 | + | 220793 | 1.12 | 0.004008 |
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Target: 5'- gGCGCCCUGGAUGUAGCUCUCGUACUCg -3' miRNA: 3'- -CGCGGGACCUACAUCGAGAGCAUGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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