Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 14616 | 5' | -55.9 | NC_003521.1 | + | 57098 | 0.67 | 0.923923 |
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Target: 5'- gAUgGCCGGGUaGAGGCCccagcagccggccaGCUCcacgaagcgauagGUCUCg -3' miRNA: 3'- -UAgUGGUCCAaCUCCGG--------------CGAG-------------UAGAG- -5' |
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| 14616 | 5' | -55.9 | NC_003521.1 | + | 34660 | 0.67 | 0.926061 |
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Target: 5'- -cCcCCAGGUcgGAGGCCaGCUCGUa-- -3' miRNA: 3'- uaGuGGUCCAa-CUCCGG-CGAGUAgag -5' |
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| 14616 | 5' | -55.9 | NC_003521.1 | + | 151643 | 0.66 | 0.936191 |
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Target: 5'- -cCGCCGgcccGGUUc--GCCGCUCGUCUCc -3' miRNA: 3'- uaGUGGU----CCAAcucCGGCGAGUAGAG- -5' |
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| 14616 | 5' | -55.9 | NC_003521.1 | + | 225985 | 0.66 | 0.940906 |
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Target: 5'- -gCGCCGcacguaguGGUUGGGGUCGUcCAUCUg -3' miRNA: 3'- uaGUGGU--------CCAACUCCGGCGaGUAGAg -5' |
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| 14616 | 5' | -55.9 | NC_003521.1 | + | 123354 | 0.66 | 0.94539 |
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Target: 5'- cGUCGCCGGGUguggagGAGGCgGCgggggCUg -3' miRNA: 3'- -UAGUGGUCCAa-----CUCCGgCGagua-GAg -5' |
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| 14616 | 5' | -55.9 | NC_003521.1 | + | 193336 | 0.66 | 0.949645 |
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Target: 5'- gGUCGCCuucGUucccaUGucGCCGCUCGUCUUu -3' miRNA: 3'- -UAGUGGuc-CA-----ACucCGGCGAGUAGAG- -5' |
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| 14616 | 5' | -55.9 | NC_003521.1 | + | 54149 | 0.66 | 0.953673 |
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Target: 5'- -cCGCCGGcGUUggaGAGGCCGC-CGcCUCc -3' miRNA: 3'- uaGUGGUC-CAA---CUCCGGCGaGUaGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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