Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 14633 | 5' | -52.1 | NC_003521.1 | + | 54637 | 0.66 | 0.997242 |
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Target: 5'- cACGGCCGAguuCUCGCUcucgcgcacGAUGUCcUGg -3' miRNA: 3'- -UGCCGGCU---GAGCGGcuu------UUACAGaAC- -5' |
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| 14633 | 5' | -52.1 | NC_003521.1 | + | 53170 | 0.71 | 0.917703 |
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Target: 5'- cGCGGCCGccCUCGCCGAugaAGAUGg---- -3' miRNA: 3'- -UGCCGGCu-GAGCGGCU---UUUACagaac -5' |
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| 14633 | 5' | -52.1 | NC_003521.1 | + | 52759 | 0.66 | 0.996211 |
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Target: 5'- -aGGCCGcACUCGUuaaccaggaUGGAGAUGUCc-- -3' miRNA: 3'- ugCCGGC-UGAGCG---------GCUUUUACAGaac -5' |
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| 14633 | 5' | -52.1 | NC_003521.1 | + | 50450 | 0.69 | 0.974785 |
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Target: 5'- aGCGGUgGACggaGCCGAAAgccgcGUGUCggUGa -3' miRNA: 3'- -UGCCGgCUGag-CGGCUUU-----UACAGa-AC- -5' |
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| 14633 | 5' | -52.1 | NC_003521.1 | + | 44460 | 0.68 | 0.987037 |
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Target: 5'- gGCGGUgGAagugUUCGCCGAGAAccUGUCc-- -3' miRNA: 3'- -UGCCGgCU----GAGCGGCUUUU--ACAGaac -5' |
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| 14633 | 5' | -52.1 | NC_003521.1 | + | 43867 | 0.67 | 0.992208 |
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Target: 5'- cCGGCCGGCUC-CCGGGcc-GUCg-- -3' miRNA: 3'- uGCCGGCUGAGcGGCUUuuaCAGaac -5' |
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| 14633 | 5' | -52.1 | NC_003521.1 | + | 34223 | 0.69 | 0.966186 |
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Target: 5'- gACGGCgccCGugUCGCCGcAGAGgaUCUUGa -3' miRNA: 3'- -UGCCG---GCugAGCGGC-UUUUacAGAAC- -5' |
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| 14633 | 5' | -52.1 | NC_003521.1 | + | 27570 | 0.67 | 0.990987 |
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Target: 5'- gGCGGUCGACgCGUCGGAcuccgauGcgGUCUg- -3' miRNA: 3'- -UGCCGGCUGaGCGGCUU-------UuaCAGAac -5' |
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| 14633 | 5' | -52.1 | NC_003521.1 | + | 23844 | 0.66 | 0.994091 |
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Target: 5'- -aGGCCGACcUGCUGGcgcuGUGUCUc- -3' miRNA: 3'- ugCCGGCUGaGCGGCUuu--UACAGAac -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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