Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1494 | 3' | -54.4 | NC_001335.1 | + | 34251 | 0.67 | 0.690114 |
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Target: 5'- -cGA-CCUCCAUCgcggCGAUgCCGucGAGCg -3' miRNA: 3'- guCUgGGAGGUAGa---GCUAgGGC--UUCG- -5' |
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| 1494 | 3' | -54.4 | NC_001335.1 | + | 39593 | 0.69 | 0.569028 |
|
Target: 5'- -cGGCCCUgaCCAUCUCGAugUUgCGGAGg -3' miRNA: 3'- guCUGGGA--GGUAGAGCU--AGgGCUUCg -5' |
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| 1494 | 3' | -54.4 | NC_001335.1 | + | 40087 | 0.7 | 0.494394 |
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Target: 5'- uGGA-CCUUgGUCUCGAUCaCgCGAGGCu -3' miRNA: 3'- gUCUgGGAGgUAGAGCUAG-G-GCUUCG- -5' |
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| 1494 | 3' | -54.4 | NC_001335.1 | + | 40124 | 0.66 | 0.768553 |
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Target: 5'- cCGGGCUCUCCGggUCGAUCCgcuuguggguccagaCGGucGGCu -3' miRNA: 3'- -GUCUGGGAGGUagAGCUAGG---------------GCU--UCG- -5' |
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| 1494 | 3' | -54.4 | NC_001335.1 | + | 46852 | 0.66 | 0.774635 |
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Target: 5'- -cGACCUgggCCAUCUCcagCCCGuacuuGGCc -3' miRNA: 3'- guCUGGGa--GGUAGAGcuaGGGCu----UCG- -5' |
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| 1494 | 3' | -54.4 | NC_001335.1 | + | 50908 | 0.69 | 0.590928 |
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Target: 5'- uCAGACCCuucUCCAgcUCgCGAUCCaacagacgCGGAGCc -3' miRNA: 3'- -GUCUGGG---AGGU--AGaGCUAGG--------GCUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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