miRNA display CGI


Results 21 - 26 of 26 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
1494 3' -54.4 NC_001335.1 + 25167 0.66 0.763444
Target:  5'- gGGuCCagguCUCCAUCUCGAUgagccgcuucucgCCCGcGAGCc -3'
miRNA:   3'- gUCuGG----GAGGUAGAGCUA-------------GGGC-UUCG- -5'
1494 3' -54.4 NC_001335.1 + 7651 0.66 0.764469
Target:  5'- gCAGGCCCUCgCGgagcuUCUCGAugcccuUCUCGAAu- -3'
miRNA:   3'- -GUCUGGGAG-GU-----AGAGCU------AGGGCUUcg -5'
1494 3' -54.4 NC_001335.1 + 19951 0.66 0.764469
Target:  5'- uCGGACCCaUCCA-CUCGuugauGUCCgUGggGUc -3'
miRNA:   3'- -GUCUGGG-AGGUaGAGC-----UAGG-GCuuCG- -5'
1494 3' -54.4 NC_001335.1 + 14577 0.66 0.764469
Target:  5'- gGGAUCCUCgGUggUGAUCUcgCGGAGCu -3'
miRNA:   3'- gUCUGGGAGgUAgaGCUAGG--GCUUCG- -5'
1494 3' -54.4 NC_001335.1 + 40124 0.66 0.768553
Target:  5'- cCGGGCUCUCCGggUCGAUCCgcuuguggguccagaCGGucGGCu -3'
miRNA:   3'- -GUCUGGGAGGUagAGCUAGG---------------GCU--UCG- -5'
1494 3' -54.4 NC_001335.1 + 46852 0.66 0.774635
Target:  5'- -cGACCUgggCCAUCUCcagCCCGuacuuGGCc -3'
miRNA:   3'- guCUGGGa--GGUAGAGcuaGGGCu----UCG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.