Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1494 | 3' | -54.4 | NC_001335.1 | + | 13372 | 0.68 | 0.657173 |
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Target: 5'- gUAGACCUUCUcgGUCUCGAaccccucguugUCCUGcucAGCa -3' miRNA: 3'- -GUCUGGGAGG--UAGAGCU-----------AGGGCu--UCG- -5' |
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| 1494 | 3' | -54.4 | NC_001335.1 | + | 4957 | 0.68 | 0.645026 |
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Target: 5'- gUAGACCCcgccgccUCCGUgCUgGAgCCCGAcgAGCa -3' miRNA: 3'- -GUCUGGG-------AGGUA-GAgCUaGGGCU--UCG- -5' |
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| 1494 | 3' | -54.4 | NC_001335.1 | + | 16518 | 0.69 | 0.569028 |
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Target: 5'- -cGACCCggCCAUgUucaCGAggCCCGAGGCc -3' miRNA: 3'- guCUGGGa-GGUAgA---GCUa-GGGCUUCG- -5' |
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| 1494 | 3' | -54.4 | NC_001335.1 | + | 7608 | 0.7 | 0.547334 |
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Target: 5'- gGGAUCCUCCuucugcggggggAUCUCGGagaCCGggGUg -3' miRNA: 3'- gUCUGGGAGG------------UAGAGCUag-GGCuuCG- -5' |
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| 1494 | 3' | -54.4 | NC_001335.1 | + | 26662 | 0.7 | 0.522722 |
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Target: 5'- aAGGCCgUUCggCUCGGUCCCcggcugauccgcguGGAGCg -3' miRNA: 3'- gUCUGGgAGGuaGAGCUAGGG--------------CUUCG- -5' |
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| 1494 | 3' | -54.4 | NC_001335.1 | + | 7651 | 0.66 | 0.764469 |
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Target: 5'- gCAGGCCCUCgCGgagcuUCUCGAugcccuUCUCGAAu- -3' miRNA: 3'- -GUCUGGGAG-GU-----AGAGCU------AGGGCUUcg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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