Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1494 | 3' | -54.4 | NC_001335.1 | + | 39593 | 0.69 | 0.569028 |
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Target: 5'- -cGGCCCUgaCCAUCUCGAugUUgCGGAGg -3' miRNA: 3'- guCUGGGA--GGUAGAGCU--AGgGCUUCg -5' |
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| 1494 | 3' | -54.4 | NC_001335.1 | + | 7608 | 0.7 | 0.547334 |
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Target: 5'- gGGAUCCUCCuucugcggggggAUCUCGGagaCCGggGUg -3' miRNA: 3'- gUCUGGGAGG------------UAGAGCUag-GGCuuCG- -5' |
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| 1494 | 3' | -54.4 | NC_001335.1 | + | 26662 | 0.7 | 0.522722 |
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Target: 5'- aAGGCCgUUCggCUCGGUCCCcggcugauccgcguGGAGCg -3' miRNA: 3'- gUCUGGgAGGuaGAGCUAGGG--------------CUUCG- -5' |
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| 1494 | 3' | -54.4 | NC_001335.1 | + | 40087 | 0.7 | 0.494394 |
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Target: 5'- uGGA-CCUUgGUCUCGAUCaCgCGAGGCu -3' miRNA: 3'- gUCUgGGAGgUAGAGCUAG-G-GCUUCG- -5' |
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| 1494 | 3' | -54.4 | NC_001335.1 | + | 7309 | 0.71 | 0.453781 |
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Target: 5'- gCGGuuACCCUCCAUCgacgCGGggCUCGAGGUc -3' miRNA: 3'- -GUC--UGGGAGGUAGa---GCUa-GGGCUUCG- -5' |
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| 1494 | 3' | -54.4 | NC_001335.1 | + | 30101 | 1.13 | 0.000678 |
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Target: 5'- aCAGACCCUCCAUCUCGAUCCCGAAGCa -3' miRNA: 3'- -GUCUGGGAGGUAGAGCUAGGGCUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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