Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1506 | 5' | -65.6 | NC_001335.1 | + | 5871 | 0.7 | 0.122227 |
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Target: 5'- aCGGCacaGGCCAGCCCGacccGCUCggGCCu- -3' miRNA: 3'- -GCCGg--CCGGUCGGGCgc--UGAG--CGGuu -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 22584 | 0.71 | 0.098701 |
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Target: 5'- gGuGCCGGCCAGCUCGCcGAUaCGCUc- -3' miRNA: 3'- gC-CGGCCGGUCGGGCG-CUGaGCGGuu -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 1876 | 0.71 | 0.093273 |
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Target: 5'- aCGGCCGGggaauacgucCCAGCUCgacaucaGCGAgUCGCCGAu -3' miRNA: 3'- -GCCGGCC----------GGUCGGG-------CGCUgAGCGGUU- -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 30095 | 0.71 | 0.088607 |
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Target: 5'- aGGCCGGCgGGUUCGgGcACUCGCuCGAa -3' miRNA: 3'- gCCGGCCGgUCGGGCgC-UGAGCG-GUU- -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 35928 | 0.72 | 0.081688 |
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Target: 5'- gGGagCGGCCAagGCCCGCGACUUGuuCCAGg -3' miRNA: 3'- gCCg-GCCGGU--CGGGCGCUGAGC--GGUU- -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 32157 | 0.73 | 0.073062 |
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Target: 5'- cCGGCCcgcgaacucGGCCAGCUCGuCGGCaucgaagUCGCCGAa -3' miRNA: 3'- -GCCGG---------CCGGUCGGGC-GCUG-------AGCGGUU- -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 48359 | 0.76 | 0.036869 |
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Target: 5'- uCGGCCGGCCGG-CCGCGACaagguacggcgcgagCGCCGc -3' miRNA: 3'- -GCCGGCCGGUCgGGCGCUGa--------------GCGGUu -5' |
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| 1506 | 5' | -65.6 | NC_001335.1 | + | 38288 | 1.05 | 0.000177 |
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Target: 5'- uCGGCCGGCCAGCCCGCGACUCGCCAAa -3' miRNA: 3'- -GCCGGCCGGUCGGGCGCUGAGCGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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