Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1524 | 3' | -55.7 | NC_001335.1 | + | 5682 | 0.66 | 0.675747 |
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Target: 5'- aCCAAGGGCgucgagcaggUGCUCgcggCCaggcaGAGUGCCGa -3' miRNA: 3'- cGGUUCUCG----------AUGGGa---GGg----CUUACGGC- -5' |
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| 1524 | 3' | -55.7 | NC_001335.1 | + | 27363 | 0.66 | 0.675747 |
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Target: 5'- cGCCGAG-GCcaaCCUCCCGAG-GCg- -3' miRNA: 3'- -CGGUUCuCGaugGGAGGGCUUaCGgc -5' |
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| 1524 | 3' | -55.7 | NC_001335.1 | + | 16683 | 0.66 | 0.68667 |
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Target: 5'- aGCCAGGAGCgUGCCgaa-CGAGUucgcGCCGg -3' miRNA: 3'- -CGGUUCUCG-AUGGgaggGCUUA----CGGC- -5' |
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| 1524 | 3' | -55.7 | NC_001335.1 | + | 37929 | 0.66 | 0.68667 |
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Target: 5'- gGCgAAGAcGCUGCCgaacgCCCGAaucaucccgAUGCCa -3' miRNA: 3'- -CGgUUCU-CGAUGGga---GGGCU---------UACGGc -5' |
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| 1524 | 3' | -55.7 | NC_001335.1 | + | 22520 | 0.66 | 0.696457 |
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Target: 5'- cGCCcgguGAGGGCUccccagaACUUguuccaggugUCCUGGAUGCCGg -3' miRNA: 3'- -CGG----UUCUCGA-------UGGG----------AGGGCUUACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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