Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1524 | 3' | -55.7 | NC_001335.1 | + | 18142 | 0.67 | 0.598711 |
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Target: 5'- aGCCcGGAgGCUGCCUUCaccaaCGggUcuucGCCGg -3' miRNA: 3'- -CGGuUCU-CGAUGGGAGg----GCuuA----CGGC- -5' |
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| 1524 | 3' | -55.7 | NC_001335.1 | + | 28659 | 0.67 | 0.587745 |
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Target: 5'- cGCCGAGuGCgccACCUUCCCGcagGCg- -3' miRNA: 3'- -CGGUUCuCGa--UGGGAGGGCuuaCGgc -5' |
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| 1524 | 3' | -55.7 | NC_001335.1 | + | 2622 | 0.68 | 0.565932 |
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Target: 5'- cGCCGucGGGCUACgCC-CUCGGcgGCUGa -3' miRNA: 3'- -CGGUu-CUCGAUG-GGaGGGCUuaCGGC- -5' |
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| 1524 | 3' | -55.7 | NC_001335.1 | + | 12408 | 0.68 | 0.565932 |
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Target: 5'- cCCGaaGGAGCc-CCCUCCCGGuggAUGUCa -3' miRNA: 3'- cGGU--UCUCGauGGGAGGGCU---UACGGc -5' |
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| 1524 | 3' | -55.7 | NC_001335.1 | + | 22520 | 0.66 | 0.696457 |
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Target: 5'- cGCCcgguGAGGGCUccccagaACUUguuccaggugUCCUGGAUGCCGg -3' miRNA: 3'- -CGG----UUCUCGA-------UGGG----------AGGGCUUACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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