miRNA: 3'- -CGGUUCuCGaugGGAGGGCUUaCGgc -5'
miRNA: 3'- -CGGUUCuCGa--UGGGAGGGCuuaCGgc -5'
miRNA: 3'- -CGgUUCU-CGAUGGga---GGGCU---------UACGGc -5'
miRNA: 3'- -CGGUUCu-CGAUG-GGag-GGCUUa-----CGGC- -5'
miRNA: 3'- -CGGUUCUCGAUGGGAGGGCUUACGGC- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1524 | 3' | -55.7 | NC_001335.1 | + | 27363 | 0.66 | 0.675747 |
|
Target: 5'- cGCCGAG-GCcaaCCUCCCGAG-GCg- -3' miRNA: 3'- -CGGUUCuCGaugGGAGGGCUUaCGgc -5' |
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| 1524 | 3' | -55.7 | NC_001335.1 | + | 28659 | 0.67 | 0.587745 |
|
Target: 5'- cGCCGAGuGCgccACCUUCCCGcagGCg- -3' miRNA: 3'- -CGGUUCuCGa--UGGGAGGGCuuaCGgc -5' |
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| 1524 | 3' | -55.7 | NC_001335.1 | + | 37929 | 0.66 | 0.68667 |
|
Target: 5'- gGCgAAGAcGCUGCCgaacgCCCGAaucaucccgAUGCCa -3' miRNA: 3'- -CGgUUCU-CGAUGGga---GGGCU---------UACGGc -5' |
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| 1524 | 3' | -55.7 | NC_001335.1 | + | 47015 | 0.66 | 0.642771 |
|
Target: 5'- cGCCAAGcuGCUGCgCCaggCCGAAgacgugGCCGg -3' miRNA: 3'- -CGGUUCu-CGAUG-GGag-GGCUUa-----CGGC- -5' |
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| 1524 | 3' | -55.7 | NC_001335.1 | + | 51161 | 1.12 | 0.000556 |
|
Target: 5'- cGCCAAGAGCUACCCUCCCGAAUGCCGa -3' miRNA: 3'- -CGGUUCUCGAUGGGAGGGCUUACGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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