Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1548 | 3' | -44.9 | NC_001347.2 | + | 184984 | 0.66 | 1 |
Target: 5'- gGGCUGCAGGcggcagcggauACUgGCCGUgacuugcuggaUCuCGUGGg -3' miRNA: 3'- -CCGAUGUUU-----------UGA-UGGCAaa---------AG-GCACC- -5' |
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1548 | 3' | -44.9 | NC_001347.2 | + | 6206 | 0.66 | 1 |
Target: 5'- cGGCgGCGAAACaaccagcGCCGggUaCUGUGGc -3' miRNA: 3'- -CCGaUGUUUUGa------UGGCaaAaGGCACC- -5' |
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1548 | 3' | -44.9 | NC_001347.2 | + | 149558 | 0.67 | 1 |
Target: 5'- uGCUACGAGACggggggaaacACCG----CCGUGGa -3' miRNA: 3'- cCGAUGUUUUGa---------UGGCaaaaGGCACC- -5' |
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1548 | 3' | -44.9 | NC_001347.2 | + | 59801 | 0.67 | 1 |
Target: 5'- -uCUACGAGACgGCCGcgaaggccuacgCCGUGGg -3' miRNA: 3'- ccGAUGUUUUGaUGGCaaaa--------GGCACC- -5' |
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1548 | 3' | -44.9 | NC_001347.2 | + | 14514 | 0.67 | 1 |
Target: 5'- aGGCggcguCAAAGCUcaucuguugccgcgaGCCGUUgacgCCGUuGGg -3' miRNA: 3'- -CCGau---GUUUUGA---------------UGGCAAaa--GGCA-CC- -5' |
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1548 | 3' | -44.9 | NC_001347.2 | + | 100537 | 0.67 | 1 |
Target: 5'- cGGCccgcgACAAAACUGCgucguagguaccagaUGUUcaCCGUGGc -3' miRNA: 3'- -CCGa----UGUUUUGAUG---------------GCAAaaGGCACC- -5' |
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1548 | 3' | -44.9 | NC_001347.2 | + | 24589 | 0.67 | 1 |
Target: 5'- cGCUcaGCGAAaccggcacccgcGCUGCCGUcgUCCGUu- -3' miRNA: 3'- cCGA--UGUUU------------UGAUGGCAaaAGGCAcc -5' |
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1548 | 3' | -44.9 | NC_001347.2 | + | 93819 | 0.67 | 1 |
Target: 5'- aGGUUACuGAAuCUGCCGUcg-CCGUucgcGGa -3' miRNA: 3'- -CCGAUGuUUU-GAUGGCAaaaGGCA----CC- -5' |
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1548 | 3' | -44.9 | NC_001347.2 | + | 92237 | 0.66 | 1 |
Target: 5'- aGCUGCAAcccgcCUGCCcgcaggCCGUGGc -3' miRNA: 3'- cCGAUGUUuu---GAUGGcaaaa-GGCACC- -5' |
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1548 | 3' | -44.9 | NC_001347.2 | + | 101508 | 0.66 | 1 |
Target: 5'- uGCUacGCAAGGCgccGCCGUacUuuGUGGu -3' miRNA: 3'- cCGA--UGUUUUGa--UGGCAaaAggCACC- -5' |
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1548 | 3' | -44.9 | NC_001347.2 | + | 126662 | 0.66 | 1 |
Target: 5'- cGGUUGCucGAgUACCGg---CgCGUGGu -3' miRNA: 3'- -CCGAUGuuUUgAUGGCaaaaG-GCACC- -5' |
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1548 | 3' | -44.9 | NC_001347.2 | + | 103573 | 0.67 | 0.999999 |
Target: 5'- uGCUGCAggccugucguGAACUguuuuugGCCGUgcaaUUCGUGGg -3' miRNA: 3'- cCGAUGU----------UUUGA-------UGGCAaa--AGGCACC- -5' |
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1548 | 3' | -44.9 | NC_001347.2 | + | 29301 | 0.67 | 0.999999 |
Target: 5'- uGGCUcCGGGACUAUCGUgUUCCc--- -3' miRNA: 3'- -CCGAuGUUUUGAUGGCAaAAGGcacc -5' |
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1548 | 3' | -44.9 | NC_001347.2 | + | 140330 | 0.67 | 0.999999 |
Target: 5'- gGGCUGCGccGCgggGCUGUgcgaggcCUGUGGg -3' miRNA: 3'- -CCGAUGUuuUGa--UGGCAaaa----GGCACC- -5' |
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1548 | 3' | -44.9 | NC_001347.2 | + | 96510 | 0.68 | 0.999998 |
Target: 5'- gGGCcgcgACAAGGC-GCUGg---CCGUGGa -3' miRNA: 3'- -CCGa---UGUUUUGaUGGCaaaaGGCACC- -5' |
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1548 | 3' | -44.9 | NC_001347.2 | + | 181223 | 0.68 | 0.999997 |
Target: 5'- cGGCUACGAccccgccauggacGccguCUACCGUUUggCCGaGGa -3' miRNA: 3'- -CCGAUGUU-------------Uu---GAUGGCAAAa-GGCaCC- -5' |
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1548 | 3' | -44.9 | NC_001347.2 | + | 79335 | 0.68 | 0.999997 |
Target: 5'- uGGCUGCAcacggagcacGGGCUGCUGgucUCCaUGGc -3' miRNA: 3'- -CCGAUGU----------UUUGAUGGCaaaAGGcACC- -5' |
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1548 | 3' | -44.9 | NC_001347.2 | + | 149885 | 0.68 | 0.999996 |
Target: 5'- cGGC-GCAGGGCgGCCGUUucUUuuuacacagccccgCCGUGGg -3' miRNA: 3'- -CCGaUGUUUUGaUGGCAA--AA--------------GGCACC- -5' |
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1548 | 3' | -44.9 | NC_001347.2 | + | 194083 | 0.69 | 0.999995 |
Target: 5'- cGCUACGAGAgcAUCGgcc-CCGUGGa -3' miRNA: 3'- cCGAUGUUUUgaUGGCaaaaGGCACC- -5' |
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1548 | 3' | -44.9 | NC_001347.2 | + | 65178 | 0.69 | 0.999995 |
Target: 5'- cGGCUACAGcgagugcggcgauGGCUcGCCGg---CCGUGu -3' miRNA: 3'- -CCGAUGUU-------------UUGA-UGGCaaaaGGCACc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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