Results 1 - 20 of 52 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1554 | 5' | -64.1 | NC_001347.2 | + | 182169 | 0.66 | 0.685233 |
Target: 5'- cGGGuCCcuggaAGCGUgugugGGCCGGCccucggggugcggGUGGCGg -3' miRNA: 3'- -CCCuGGcg---UCGCA-----CCGGCCG-------------CACCGC- -5' |
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1554 | 5' | -64.1 | NC_001347.2 | + | 1843 | 0.66 | 0.676875 |
Target: 5'- cGGcGCCGCAccccGCGUcgcugcugacGGCCGuGCGUcGGCa -3' miRNA: 3'- -CCcUGGCGU----CGCA----------CCGGC-CGCA-CCGc -5' |
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1554 | 5' | -64.1 | NC_001347.2 | + | 2094 | 0.66 | 0.676875 |
Target: 5'- --aGCCGUacGGCGccGCCGGCGggGGCGc -3' miRNA: 3'- cccUGGCG--UCGCacCGGCCGCa-CCGC- -5' |
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1554 | 5' | -64.1 | NC_001347.2 | + | 191638 | 0.66 | 0.674083 |
Target: 5'- -aGGCCGUcagguGCGaGGCCgaacggugaaucuuGGCGUGGCGc -3' miRNA: 3'- ccCUGGCGu----CGCaCCGG--------------CCGCACCGC- -5' |
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1554 | 5' | -64.1 | NC_001347.2 | + | 178067 | 0.66 | 0.66756 |
Target: 5'- gGGGACUGauccaGGCGUcGGCguugGGCaagGUGGCGc -3' miRNA: 3'- -CCCUGGCg----UCGCA-CCGg---CCG---CACCGC- -5' |
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1554 | 5' | -64.1 | NC_001347.2 | + | 136086 | 0.66 | 0.658221 |
Target: 5'- cGGGcCCGCGGac-GGaCCGGCGccgGGCc -3' miRNA: 3'- -CCCuGGCGUCgcaCC-GGCCGCa--CCGc -5' |
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1554 | 5' | -64.1 | NC_001347.2 | + | 155314 | 0.66 | 0.648864 |
Target: 5'- cGGGGCCG-GGC-UGGUCGGgggaagaaaCGUGGUGu -3' miRNA: 3'- -CCCUGGCgUCGcACCGGCC---------GCACCGC- -5' |
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1554 | 5' | -64.1 | NC_001347.2 | + | 7803 | 0.66 | 0.648864 |
Target: 5'- uGGGuACCGUcacuagugacguGGCGUGacgaaGCUGGUacgGUGGCGg -3' miRNA: 3'- -CCC-UGGCG------------UCGCAC-----CGGCCG---CACCGC- -5' |
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1554 | 5' | -64.1 | NC_001347.2 | + | 42 | 0.67 | 0.639496 |
Target: 5'- uGGGugUGUcggGGCGcGGCCGG-GUGGg- -3' miRNA: 3'- -CCCugGCG---UCGCaCCGGCCgCACCgc -5' |
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1554 | 5' | -64.1 | NC_001347.2 | + | 31103 | 0.67 | 0.630123 |
Target: 5'- --aACCGgAGCGUgcuccauugccGGCCGGCGUccaGGCu -3' miRNA: 3'- cccUGGCgUCGCA-----------CCGGCCGCA---CCGc -5' |
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1554 | 5' | -64.1 | NC_001347.2 | + | 95469 | 0.67 | 0.620752 |
Target: 5'- uGGGCgCGCGucacGCG-GcGCCGGUGUcGGCGg -3' miRNA: 3'- cCCUG-GCGU----CGCaC-CGGCCGCA-CCGC- -5' |
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1554 | 5' | -64.1 | NC_001347.2 | + | 181741 | 0.67 | 0.611388 |
Target: 5'- -cGACCGCuGCGUGcGCCgacGGCGUuucaaGGUGu -3' miRNA: 3'- ccCUGGCGuCGCAC-CGG---CCGCA-----CCGC- -5' |
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1554 | 5' | -64.1 | NC_001347.2 | + | 2199 | 0.67 | 0.611388 |
Target: 5'- uGGGuuCGaCAGCGaUGGCgGGUccGGCGg -3' miRNA: 3'- -CCCugGC-GUCGC-ACCGgCCGcaCCGC- -5' |
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1554 | 5' | -64.1 | NC_001347.2 | + | 77863 | 0.67 | 0.611388 |
Target: 5'- --uGCUGCAG-GUGGCCGaGCGccugGGCGc -3' miRNA: 3'- cccUGGCGUCgCACCGGC-CGCa---CCGC- -5' |
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1554 | 5' | -64.1 | NC_001347.2 | + | 138815 | 0.67 | 0.602036 |
Target: 5'- -aGACCGCAGUgGUGGCCacgGUGGUGu -3' miRNA: 3'- ccCUGGCGUCG-CACCGGccgCACCGC- -5' |
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1554 | 5' | -64.1 | NC_001347.2 | + | 138223 | 0.67 | 0.592704 |
Target: 5'- gGGGugUGUAGCuUGuaCGGCGUuuugucGGCGg -3' miRNA: 3'- -CCCugGCGUCGcACcgGCCGCA------CCGC- -5' |
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1554 | 5' | -64.1 | NC_001347.2 | + | 117898 | 0.68 | 0.583395 |
Target: 5'- cGGGGCgGCGGCG-GGuCCcGUGaUGGCa -3' miRNA: 3'- -CCCUGgCGUCGCaCC-GGcCGC-ACCGc -5' |
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1554 | 5' | -64.1 | NC_001347.2 | + | 104337 | 0.68 | 0.582466 |
Target: 5'- uGGACCGCuggauccGGCacgcGGCCGGCGUagaacGGCc -3' miRNA: 3'- cCCUGGCG-------UCGca--CCGGCCGCA-----CCGc -5' |
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1554 | 5' | -64.1 | NC_001347.2 | + | 190143 | 0.68 | 0.574117 |
Target: 5'- cGGGGCCGCAaccGgGUucuGCCGGCaUGGgGg -3' miRNA: 3'- -CCCUGGCGU---CgCAc--CGGCCGcACCgC- -5' |
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1554 | 5' | -64.1 | NC_001347.2 | + | 13923 | 0.68 | 0.559344 |
Target: 5'- gGGGcgucggagaguauaaACUGgGGCG-GGCgCGGgGUGGCGa -3' miRNA: 3'- -CCC---------------UGGCgUCGCaCCG-GCCgCACCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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