Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15658 | 5' | -55.7 | NC_004065.1 | + | 1427 | 0.66 | 0.938985 |
Target: 5'- -gGCAGCGGCGCCgaCgacacaauGGGGACc- -3' miRNA: 3'- uaCGUUGUCGCGGgaGau------CUCCUGuu -5' |
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15658 | 5' | -55.7 | NC_004065.1 | + | 22482 | 0.66 | 0.938985 |
Target: 5'- uUGUAGuCGaCGUCCUUUGGAGGGCGu -3' miRNA: 3'- uACGUU-GUcGCGGGAGAUCUCCUGUu -5' |
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15658 | 5' | -55.7 | NC_004065.1 | + | 191280 | 0.66 | 0.934087 |
Target: 5'- aGUGCAcuuCGGgGCCCUCUcuGAGGcggaGCAGg -3' miRNA: 3'- -UACGUu--GUCgCGGGAGAu-CUCC----UGUU- -5' |
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15658 | 5' | -55.7 | NC_004065.1 | + | 39619 | 0.66 | 0.934087 |
Target: 5'- aGUGCuACcGgGCCCUCcgAGGGGAgGAg -3' miRNA: 3'- -UACGuUGuCgCGGGAGa-UCUCCUgUU- -5' |
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15658 | 5' | -55.7 | NC_004065.1 | + | 23046 | 0.66 | 0.934086 |
Target: 5'- -aGCGACGGUGgCCggucguGAGGACGAc -3' miRNA: 3'- uaCGUUGUCGCgGGagau--CUCCUGUU- -5' |
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15658 | 5' | -55.7 | NC_004065.1 | + | 41372 | 0.66 | 0.923564 |
Target: 5'- -cGCcGC-GCGCCCUCgAGcGGACAu -3' miRNA: 3'- uaCGuUGuCGCGGGAGaUCuCCUGUu -5' |
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15658 | 5' | -55.7 | NC_004065.1 | + | 122590 | 0.66 | 0.923564 |
Target: 5'- cGUGCGGaAGCugGCCCU--GGAGGACAGc -3' miRNA: 3'- -UACGUUgUCG--CGGGAgaUCUCCUGUU- -5' |
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15658 | 5' | -55.7 | NC_004065.1 | + | 199806 | 0.66 | 0.91794 |
Target: 5'- -cGCGGCAcgauggggcGCGCCCggCUGGAGG-CGc -3' miRNA: 3'- uaCGUUGU---------CGCGGGa-GAUCUCCuGUu -5' |
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15658 | 5' | -55.7 | NC_004065.1 | + | 124959 | 0.66 | 0.91794 |
Target: 5'- -cGCGGCAGCGCCUgcacgaAGGGCGg -3' miRNA: 3'- uaCGUUGUCGCGGGagauc-UCCUGUu -5' |
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15658 | 5' | -55.7 | NC_004065.1 | + | 99374 | 0.66 | 0.912074 |
Target: 5'- -aGCGACGGCGCCgaCUGucccgacucGAGcGACAGg -3' miRNA: 3'- uaCGUUGUCGCGGgaGAU---------CUC-CUGUU- -5' |
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15658 | 5' | -55.7 | NC_004065.1 | + | 47 | 0.67 | 0.905969 |
Target: 5'- gAUGgGGgGGCuauauuuuuGUCCUCUAGAGGGCGc -3' miRNA: 3'- -UACgUUgUCG---------CGGGAGAUCUCCUGUu -5' |
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15658 | 5' | -55.7 | NC_004065.1 | + | 115060 | 0.67 | 0.905969 |
Target: 5'- -gGCAACGGCGCgCgc--GAGGACGc -3' miRNA: 3'- uaCGUUGUCGCGgGagauCUCCUGUu -5' |
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15658 | 5' | -55.7 | NC_004065.1 | + | 27774 | 0.67 | 0.905969 |
Target: 5'- -cGCuGCGcCGCCgUCUGGAGGGCc- -3' miRNA: 3'- uaCGuUGUcGCGGgAGAUCUCCUGuu -5' |
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15658 | 5' | -55.7 | NC_004065.1 | + | 89705 | 0.67 | 0.905969 |
Target: 5'- -cGCAgaGCAGCGUCUUCUucaAGaAGGGCGc -3' miRNA: 3'- uaCGU--UGUCGCGGGAGA---UC-UCCUGUu -5' |
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15658 | 5' | -55.7 | NC_004065.1 | + | 68348 | 0.67 | 0.899626 |
Target: 5'- cUGCAGCAGCuGCCCg-UGGAGaACAc -3' miRNA: 3'- uACGUUGUCG-CGGGagAUCUCcUGUu -5' |
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15658 | 5' | -55.7 | NC_004065.1 | + | 26423 | 0.67 | 0.899626 |
Target: 5'- -gGCGGCGGCGgCCUCcgAGAGcACAu -3' miRNA: 3'- uaCGUUGUCGCgGGAGa-UCUCcUGUu -5' |
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15658 | 5' | -55.7 | NC_004065.1 | + | 19581 | 0.67 | 0.899625 |
Target: 5'- gAUGUAGCGuGCGUCCUCcgGGuAGGAUGAc -3' miRNA: 3'- -UACGUUGU-CGCGGGAGa-UC-UCCUGUU- -5' |
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15658 | 5' | -55.7 | NC_004065.1 | + | 127778 | 0.67 | 0.893048 |
Target: 5'- -gGCGACGGgGCCCaugagcaggCggcAGAGGACGAc -3' miRNA: 3'- uaCGUUGUCgCGGGa--------Ga--UCUCCUGUU- -5' |
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15658 | 5' | -55.7 | NC_004065.1 | + | 221906 | 0.68 | 0.864463 |
Target: 5'- --uCGACGGCGCCCggcUCUGGGGcGACu- -3' miRNA: 3'- uacGUUGUCGCGGG---AGAUCUC-CUGuu -5' |
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15658 | 5' | -55.7 | NC_004065.1 | + | 217091 | 0.68 | 0.856773 |
Target: 5'- --aCAGCGGCGUCCgUCUcuguAGAGGAUAAu -3' miRNA: 3'- uacGUUGUCGCGGG-AGA----UCUCCUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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