Results 1 - 20 of 39 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
15676 | 3' | -54.2 | NC_004065.1 | + | 32283 | 0.66 | 0.980675 |
Target: 5'- cGCUGUccGCGUCCUCGGUcuccUCGUCGg -3' miRNA: 3'- -CGGUAccUGCAGGAGCCGu---AGUAGUa -5' |
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15676 | 3' | -54.2 | NC_004065.1 | + | 73268 | 0.66 | 0.980675 |
Target: 5'- ----aGGGCGUCUcgcucguggCGGCAUCGUCGc -3' miRNA: 3'- cgguaCCUGCAGGa--------GCCGUAGUAGUa -5' |
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15676 | 3' | -54.2 | NC_004065.1 | + | 125851 | 0.66 | 0.978485 |
Target: 5'- gGUCGUGGGCagcugGUUCUCGGCcgUGUCc- -3' miRNA: 3'- -CGGUACCUG-----CAGGAGCCGuaGUAGua -5' |
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15676 | 3' | -54.2 | NC_004065.1 | + | 206541 | 0.66 | 0.978485 |
Target: 5'- ----cGGACGcgaaucggaCUCGGCGUCGUCAUc -3' miRNA: 3'- cgguaCCUGCag-------GAGCCGUAGUAGUA- -5' |
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15676 | 3' | -54.2 | NC_004065.1 | + | 118583 | 0.66 | 0.978485 |
Target: 5'- cGCCAUGGACGUCggCGGUccCGc--- -3' miRNA: 3'- -CGGUACCUGCAGgaGCCGuaGUagua -5' |
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15676 | 3' | -54.2 | NC_004065.1 | + | 129693 | 0.66 | 0.973563 |
Target: 5'- cGCCA-GGGCGUCUUCG--AUUAUCAc -3' miRNA: 3'- -CGGUaCCUGCAGGAGCcgUAGUAGUa -5' |
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15676 | 3' | -54.2 | NC_004065.1 | + | 222894 | 0.66 | 0.973563 |
Target: 5'- -aCGUGGAUGUgaaagcUCUCGGCAUCGa--- -3' miRNA: 3'- cgGUACCUGCA------GGAGCCGUAGUagua -5' |
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15676 | 3' | -54.2 | NC_004065.1 | + | 19394 | 0.66 | 0.970818 |
Target: 5'- aUCGUcGuCGUCCUCGGCAUCuUCu- -3' miRNA: 3'- cGGUAcCuGCAGGAGCCGUAGuAGua -5' |
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15676 | 3' | -54.2 | NC_004065.1 | + | 38437 | 0.66 | 0.970818 |
Target: 5'- cGUCGUGGACGg---CGGCAggaggcUCAUCGg -3' miRNA: 3'- -CGGUACCUGCaggaGCCGU------AGUAGUa -5' |
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15676 | 3' | -54.2 | NC_004065.1 | + | 146744 | 0.66 | 0.970818 |
Target: 5'- cGCCcacaucuauGUGGGCGUCggCGGCGUCugccgCAUc -3' miRNA: 3'- -CGG---------UACCUGCAGgaGCCGUAGua---GUA- -5' |
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15676 | 3' | -54.2 | NC_004065.1 | + | 135404 | 0.67 | 0.964724 |
Target: 5'- cGUCAUcGACGUCCcCGuCGUCAUCAc -3' miRNA: 3'- -CGGUAcCUGCAGGaGCcGUAGUAGUa -5' |
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15676 | 3' | -54.2 | NC_004065.1 | + | 70713 | 0.67 | 0.964724 |
Target: 5'- cGCCGUGaaGGUGUCCUCGGguuugAUCGUCAc -3' miRNA: 3'- -CGGUAC--CUGCAGGAGCCg----UAGUAGUa -5' |
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15676 | 3' | -54.2 | NC_004065.1 | + | 224382 | 0.67 | 0.961364 |
Target: 5'- uCCAUGuGGCGgcguUCCgUCGGCGUCAcCGUg -3' miRNA: 3'- cGGUAC-CUGC----AGG-AGCCGUAGUaGUA- -5' |
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15676 | 3' | -54.2 | NC_004065.1 | + | 26017 | 0.68 | 0.949976 |
Target: 5'- aCCGUGG----CCUCGGCGUCGUCc- -3' miRNA: 3'- cGGUACCugcaGGAGCCGUAGUAGua -5' |
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15676 | 3' | -54.2 | NC_004065.1 | + | 91450 | 0.68 | 0.949976 |
Target: 5'- gGCC-UGGACGccgaagcggcgCCU-GGCAUCGUCAc -3' miRNA: 3'- -CGGuACCUGCa----------GGAgCCGUAGUAGUa -5' |
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15676 | 3' | -54.2 | NC_004065.1 | + | 224879 | 0.68 | 0.949976 |
Target: 5'- aGCCAUccGGAcgcguacaCGUCCaCGGUGUCGUCGg -3' miRNA: 3'- -CGGUA--CCU--------GCAGGaGCCGUAGUAGUa -5' |
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15676 | 3' | -54.2 | NC_004065.1 | + | 168636 | 0.68 | 0.949976 |
Target: 5'- uGCCGcGGGCGgagCCggcgCGGCGaCGUCGUc -3' miRNA: 3'- -CGGUaCCUGCa--GGa---GCCGUaGUAGUA- -5' |
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15676 | 3' | -54.2 | NC_004065.1 | + | 39106 | 0.68 | 0.949976 |
Target: 5'- gGCgAUGGGCGUCCcgCGGCAg------ -3' miRNA: 3'- -CGgUACCUGCAGGa-GCCGUaguagua -5' |
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15676 | 3' | -54.2 | NC_004065.1 | + | 191718 | 0.68 | 0.94573 |
Target: 5'- -gCGUGGugGUgccgcaCCUCGGCGUCGa--- -3' miRNA: 3'- cgGUACCugCA------GGAGCCGUAGUagua -5' |
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15676 | 3' | -54.2 | NC_004065.1 | + | 61802 | 0.68 | 0.94573 |
Target: 5'- uGCCGUGGcaguagcagcgcACGgacUCgaCGGCGUCGUCAa -3' miRNA: 3'- -CGGUACC------------UGC---AGgaGCCGUAGUAGUa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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