Results 41 - 49 of 49 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16250 | 5' | -52 | NC_004084.1 | + | 44622 | 0.66 | 0.900528 |
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Target: 5'- uCGCCUcgGUCGCgaccaaccGGAcgCGAuCUcCGAGCCc -3' miRNA: 3'- -GCGGAa-CAGCG--------UCUa-GCU-GA-GCUUGG- -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 42131 | 0.66 | 0.900528 |
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Target: 5'- uGCCguugaggugacUGUUGCAGGgacugcacUCGACUgcaGAACCg -3' miRNA: 3'- gCGGa----------ACAGCGUCU--------AGCUGAg--CUUGG- -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 19554 | 0.66 | 0.907306 |
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Target: 5'- uGUCUUG-CGaCcGGUCGAgauccucacuCUCGAGCCa -3' miRNA: 3'- gCGGAACaGC-GuCUAGCU----------GAGCUUGG- -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 48559 | 0.66 | 0.907306 |
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Target: 5'- -aCCUcGUCGCGG-UCGAggCGAAUCa -3' miRNA: 3'- gcGGAaCAGCGUCuAGCUgaGCUUGG- -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 31402 | 0.66 | 0.911241 |
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Target: 5'- aCGCCgauaucggcgaaGUCGCAGA-CGACgCG-GCCg -3' miRNA: 3'- -GCGGaa----------CAGCGUCUaGCUGaGCuUGG- -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 26297 | 0.66 | 0.913809 |
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Target: 5'- gGUCU-GUCGacCAGAUCGugUCGuACg -3' miRNA: 3'- gCGGAaCAGC--GUCUAGCugAGCuUGg -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 40269 | 0.66 | 0.913809 |
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Target: 5'- aCGCUcg--CGCuGAUCGACcCGGACUc -3' miRNA: 3'- -GCGGaacaGCGuCUAGCUGaGCUUGG- -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 3779 | 0.66 | 0.913809 |
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Target: 5'- uGCCgUUG-CGCGGGUUGACgacgauUCGAuacgugacGCCg -3' miRNA: 3'- gCGG-AACaGCGUCUAGCUG------AGCU--------UGG- -5' |
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| 16250 | 5' | -52 | NC_004084.1 | + | 52286 | 0.66 | 0.913809 |
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Target: 5'- gGCgCUgGUCGCGGAaucgcaUCGACcUGAACg -3' miRNA: 3'- gCG-GAaCAGCGUCU------AGCUGaGCUUGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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