Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16269 | 3' | -59.6 | NC_004084.1 | + | 39331 | 0.67 | 0.450131 |
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Target: 5'- ---cGGUGCUGaUCGAcgucgacguagACGGCCGC-GCc -3' miRNA: 3'- accuCCGCGAC-AGCU-----------UGCCGGCGuCG- -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 43582 | 0.67 | 0.459722 |
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Target: 5'- -cGAGGCGCggauccuccagUGggaucgCGAguACGGCCGCuGGCc -3' miRNA: 3'- acCUCCGCG-----------ACa-----GCU--UGCCGGCG-UCG- -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 44051 | 0.66 | 0.479221 |
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Target: 5'- gGGAGGUGCg--CGAAgauGCCGCguAGCa -3' miRNA: 3'- aCCUCCGCGacaGCUUgc-CGGCG--UCG- -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 4046 | 0.66 | 0.489122 |
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Target: 5'- aGGGGGCGgaGUUGA-CGGagaaGgGGCg -3' miRNA: 3'- aCCUCCGCgaCAGCUuGCCgg--CgUCG- -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 15762 | 0.66 | 0.499118 |
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Target: 5'- gGGAGGaCGUcGUCGGACuGcGCCGCc-- -3' miRNA: 3'- aCCUCC-GCGaCAGCUUG-C-CGGCGucg -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 7360 | 0.66 | 0.499118 |
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Target: 5'- aGGAGGCGCgGuUCGcuccGACGGugaucCCGCcuGCg -3' miRNA: 3'- aCCUCCGCGaC-AGC----UUGCC-----GGCGu-CG- -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 33102 | 0.66 | 0.509203 |
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Target: 5'- aGG-GGCGCga-CG-GCGGCCucuGCGGCu -3' miRNA: 3'- aCCuCCGCGacaGCuUGCCGG---CGUCG- -5' |
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| 16269 | 3' | -59.6 | NC_004084.1 | + | 52589 | 0.66 | 0.519373 |
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Target: 5'- cGGcGGCGUgGUcCGGugGGUCGaGGCa -3' miRNA: 3'- aCCuCCGCGaCA-GCUugCCGGCgUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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