Results 1 - 20 of 30 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16566 | 5' | -49.7 | NC_004117.1 | + | 156051 | 0.66 | 0.996678 |
Target: 5'- cGCGCuuccaaccguACAUGUgcACCGACGccauuuuaGGCAAAGu -3' miRNA: 3'- aCGCGu---------UGUGCA--UGGCUGU--------UCGUUUU- -5' |
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16566 | 5' | -49.7 | NC_004117.1 | + | 54242 | 0.66 | 0.995394 |
Target: 5'- uUGCGCAGCcucgUGUACCcuuACGAGUAGGu -3' miRNA: 3'- -ACGCGUUGu---GCAUGGc--UGUUCGUUUu -5' |
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16566 | 5' | -49.7 | NC_004117.1 | + | 43218 | 0.66 | 0.995394 |
Target: 5'- cGUGCGGCugGcauCCGACAGGa---- -3' miRNA: 3'- aCGCGUUGugCau-GGCUGUUCguuuu -5' |
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16566 | 5' | -49.7 | NC_004117.1 | + | 73240 | 0.66 | 0.994614 |
Target: 5'- aUGCGCGACAaguUGUACgaggCGACAcGCAu-- -3' miRNA: 3'- -ACGCGUUGU---GCAUG----GCUGUuCGUuuu -5' |
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16566 | 5' | -49.7 | NC_004117.1 | + | 157555 | 0.66 | 0.994531 |
Target: 5'- gGUGCGuCACGUggaucaaGgUGGCAAGCGAAAa -3' miRNA: 3'- aCGCGUuGUGCA-------UgGCUGUUCGUUUU- -5' |
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16566 | 5' | -49.7 | NC_004117.1 | + | 131409 | 0.67 | 0.993731 |
Target: 5'- uUGUGCAGCACacauCUGuCGAGCGGAu -3' miRNA: 3'- -ACGCGUUGUGcau-GGCuGUUCGUUUu -5' |
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16566 | 5' | -49.7 | NC_004117.1 | + | 133481 | 0.67 | 0.993731 |
Target: 5'- uUGCGC-GCACGUGCCGuCcucGGCc--- -3' miRNA: 3'- -ACGCGuUGUGCAUGGCuGu--UCGuuuu -5' |
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16566 | 5' | -49.7 | NC_004117.1 | + | 133513 | 0.67 | 0.991618 |
Target: 5'- cGCGacaGAUACGauaugGgCGACAGGCAGAu -3' miRNA: 3'- aCGCg--UUGUGCa----UgGCUGUUCGUUUu -5' |
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16566 | 5' | -49.7 | NC_004117.1 | + | 49568 | 0.67 | 0.991618 |
Target: 5'- gUGCGCGACAUgacgGUgagcuugagacgACCGACAGGUu--- -3' miRNA: 3'- -ACGCGUUGUG----CA------------UGGCUGUUCGuuuu -5' |
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16566 | 5' | -49.7 | NC_004117.1 | + | 135661 | 0.67 | 0.988978 |
Target: 5'- gGCGUAcgccccugGCAucuauUGUGCCGACuuuAGCAAGAu -3' miRNA: 3'- aCGCGU--------UGU-----GCAUGGCUGu--UCGUUUU- -5' |
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16566 | 5' | -49.7 | NC_004117.1 | + | 55231 | 0.68 | 0.987435 |
Target: 5'- cGCGUucacucuACACGgacCCGACGAGCu--- -3' miRNA: 3'- aCGCGu------UGUGCau-GGCUGUUCGuuuu -5' |
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16566 | 5' | -49.7 | NC_004117.1 | + | 29734 | 0.68 | 0.983856 |
Target: 5'- uUGUuuuCAACAcCGUaACCGACGAGCAAc- -3' miRNA: 3'- -ACGc--GUUGU-GCA-UGGCUGUUCGUUuu -5' |
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16566 | 5' | -49.7 | NC_004117.1 | + | 141225 | 0.68 | 0.981799 |
Target: 5'- cGCGCGACACGUuUCG-CA-GCAGu- -3' miRNA: 3'- aCGCGUUGUGCAuGGCuGUuCGUUuu -5' |
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16566 | 5' | -49.7 | NC_004117.1 | + | 8294 | 0.68 | 0.979552 |
Target: 5'- cGCGCGuguacgauACACGUGCCGAUuacguGCu--- -3' miRNA: 3'- aCGCGU--------UGUGCAUGGCUGuu---CGuuuu -5' |
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16566 | 5' | -49.7 | NC_004117.1 | + | 14773 | 0.68 | 0.979552 |
Target: 5'- -uCGCuuguuACACaGgggACCGACAAGCAAAGu -3' miRNA: 3'- acGCGu----UGUG-Ca--UGGCUGUUCGUUUU- -5' |
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16566 | 5' | -49.7 | NC_004117.1 | + | 34028 | 0.68 | 0.979552 |
Target: 5'- gGCGCAuuACAUuUGCaCGAUAAGCGAc- -3' miRNA: 3'- aCGCGU--UGUGcAUG-GCUGUUCGUUuu -5' |
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16566 | 5' | -49.7 | NC_004117.1 | + | 71463 | 0.69 | 0.977106 |
Target: 5'- aUGCGCAugGCGuUGCCGGCc-GUAc-- -3' miRNA: 3'- -ACGCGUugUGC-AUGGCUGuuCGUuuu -5' |
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16566 | 5' | -49.7 | NC_004117.1 | + | 42150 | 0.69 | 0.97445 |
Target: 5'- uUGCGCGACAC--AUCGACAuuggugccuAGCAAu- -3' miRNA: 3'- -ACGCGUUGUGcaUGGCUGU---------UCGUUuu -5' |
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16566 | 5' | -49.7 | NC_004117.1 | + | 39485 | 0.7 | 0.957761 |
Target: 5'- aGUGCGA-ACGUGCCGACAcuaaugcucGCAAAAu -3' miRNA: 3'- aCGCGUUgUGCAUGGCUGUu--------CGUUUU- -5' |
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16566 | 5' | -49.7 | NC_004117.1 | + | 96896 | 0.7 | 0.957761 |
Target: 5'- cGCGuCAaagcACACGcUACCGAucCGAGCAAAu -3' miRNA: 3'- aCGC-GU----UGUGC-AUGGCU--GUUCGUUUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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