Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16570 | 3' | -46.1 | NC_004117.1 | + | 98689 | 0.66 | 0.999965 |
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Target: 5'- uGCCGgc-GCucAUG-CGGUCAGCACCUu -3' miRNA: 3'- -UGGCauuUGu-UACgGUUAGUUGUGGA- -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 107811 | 0.67 | 0.999889 |
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Target: 5'- gGuuGUAuuauauGCGGUGCCAucAUCGcCGCCUg -3' miRNA: 3'- -UggCAUu-----UGUUACGGU--UAGUuGUGGA- -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 113061 | 0.67 | 0.999811 |
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Target: 5'- cUCGUAua-AGUGCCG-UCAGCACUa -3' miRNA: 3'- uGGCAUuugUUACGGUuAGUUGUGGa -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 113642 | 0.67 | 0.999811 |
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Target: 5'- uGCCGcaggUGAACuuUGCCAAcgaCAugACCUg -3' miRNA: 3'- -UGGC----AUUUGuuACGGUUa--GUugUGGA- -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 133557 | 0.67 | 0.999756 |
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Target: 5'- uCCGUGGACAAUcaaCCAAUCGAUggcgggcaACCg -3' miRNA: 3'- uGGCAUUUGUUAc--GGUUAGUUG--------UGGa -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 134942 | 0.67 | 0.999916 |
|
Target: 5'- gAUCGUAAcCAAaGCCGAcaacgcUCGACACUUg -3' miRNA: 3'- -UGGCAUUuGUUaCGGUU------AGUUGUGGA- -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 138155 | 0.67 | 0.999756 |
|
Target: 5'- cGCCGauuguCGAcUGCCAAUCGACAUg- -3' miRNA: 3'- -UGGCauuu-GUU-ACGGUUAGUUGUGga -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 146618 | 0.7 | 0.996226 |
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Target: 5'- uGCCGUGGACGAcuuuagcauUGCCGG-CAugauuuacguuggcGCACCUa -3' miRNA: 3'- -UGGCAUUUGUU---------ACGGUUaGU--------------UGUGGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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