Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16570 | 3' | -46.1 | NC_004117.1 | + | 113061 | 0.67 | 0.999811 |
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Target: 5'- cUCGUAua-AGUGCCG-UCAGCACUa -3' miRNA: 3'- uGGCAUuugUUACGGUuAGUUGUGGa -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 45865 | 0.67 | 0.999756 |
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Target: 5'- gACCGccuugGAACGAUGCCAAcCAAUguACUg -3' miRNA: 3'- -UGGCa----UUUGUUACGGUUaGUUG--UGGa -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 138155 | 0.67 | 0.999756 |
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Target: 5'- cGCCGauuguCGAcUGCCAAUCGACAUg- -3' miRNA: 3'- -UGGCauuu-GUU-ACGGUUAGUUGUGga -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 5490 | 0.68 | 0.999501 |
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Target: 5'- cCCGUcAAACAcGUGCUGgacaucaauGUCAACACCa -3' miRNA: 3'- uGGCA-UUUGU-UACGGU---------UAGUUGUGGa -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 93055 | 0.68 | 0.999428 |
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Target: 5'- gAUUGUAAAUAGUGCCuucgccgucugguauGggCAGCACCa -3' miRNA: 3'- -UGGCAUUUGUUACGG---------------UuaGUUGUGGa -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 96404 | 0.69 | 0.998251 |
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Target: 5'- aGCCGUGcgcgauucACGGUGCCGG-CuACACCUa -3' miRNA: 3'- -UGGCAUu-------UGUUACGGUUaGuUGUGGA- -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 3854 | 0.72 | 0.99092 |
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Target: 5'- uGCCGUAGuuuc-GCCAcccGUCGACGCCg -3' miRNA: 3'- -UGGCAUUuguuaCGGU---UAGUUGUGGa -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 80362 | 0.66 | 0.999978 |
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Target: 5'- -aUGUAGuuGAUGCCAAUCuugcgcaaauaccauAACACCa -3' miRNA: 3'- ugGCAUUugUUACGGUUAG---------------UUGUGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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