Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16570 | 3' | -46.1 | NC_004117.1 | + | 107811 | 0.67 | 0.999889 |
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Target: 5'- gGuuGUAuuauauGCGGUGCCAucAUCGcCGCCUg -3' miRNA: 3'- -UggCAUu-----UGUUACGGU--UAGUuGUGGA- -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 71139 | 0.67 | 0.999889 |
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Target: 5'- -gUGUGcaaAACAAUGCCAaaGUCAACGgCg -3' miRNA: 3'- ugGCAU---UUGUUACGGU--UAGUUGUgGa -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 134942 | 0.67 | 0.999916 |
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Target: 5'- gAUCGUAAcCAAaGCCGAcaacgcUCGACACUUg -3' miRNA: 3'- -UGGCAUUuGUUaCGGUU------AGUUGUGGA- -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 28965 | 0.66 | 0.999937 |
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Target: 5'- gACCaaGAACGAggugGUCAAUCGucACACCg -3' miRNA: 3'- -UGGcaUUUGUUa---CGGUUAGU--UGUGGa -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 5959 | 0.66 | 0.999937 |
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Target: 5'- cGCCGUGAACAGcugcUGCCuccgcUAGCGCg- -3' miRNA: 3'- -UGGCAUUUGUU----ACGGuua--GUUGUGga -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 98689 | 0.66 | 0.999965 |
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Target: 5'- uGCCGgc-GCucAUG-CGGUCAGCACCUu -3' miRNA: 3'- -UGGCauuUGu-UACgGUUAGUUGUGGA- -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 38427 | 0.66 | 0.999975 |
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Target: 5'- uCCGUAAAUuuUGCCA--CGGCACa- -3' miRNA: 3'- uGGCAUUUGuuACGGUuaGUUGUGga -5' |
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| 16570 | 3' | -46.1 | NC_004117.1 | + | 80362 | 0.66 | 0.999978 |
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Target: 5'- -aUGUAGuuGAUGCCAAUCuugcgcaaauaccauAACACCa -3' miRNA: 3'- ugGCAUUugUUACGGUUAG---------------UUGUGGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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