Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16686 | 5' | -44.1 | NC_004156.1 | + | 150546 | 0.68 | 0.999999 |
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Target: 5'- aCGGUUGCACCagaauuagacgaucgCAGuAGUCGUCGGuGGa -3' miRNA: 3'- cGUUAACGUGG---------------GUU-UCAGCAGUUuUC- -5' |
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| 16686 | 5' | -44.1 | NC_004156.1 | + | 154627 | 0.66 | 1 |
|
Target: 5'- aGCAGUUGCACCUuucuGGUgG-CAGc-- -3' miRNA: 3'- -CGUUAACGUGGGuu--UCAgCaGUUuuc -5' |
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| 16686 | 5' | -44.1 | NC_004156.1 | + | 158041 | 0.67 | 1 |
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Target: 5'- cGCGAccgucaGCACCCuuGGUCGUgAAc-- -3' miRNA: 3'- -CGUUaa----CGUGGGuuUCAGCAgUUuuc -5' |
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| 16686 | 5' | -44.1 | NC_004156.1 | + | 166340 | 0.67 | 1 |
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Target: 5'- uGCAugGUUGCAUaaagUCAucGUCGUCAAu-- -3' miRNA: 3'- -CGU--UAACGUG----GGUuuCAGCAGUUuuc -5' |
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| 16686 | 5' | -44.1 | NC_004156.1 | + | 173786 | 0.68 | 0.999997 |
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Target: 5'- uGCAAUugUGCACCC-AGGUCuaggcacuuGUCAucGGa -3' miRNA: 3'- -CGUUA--ACGUGGGuUUCAG---------CAGUuuUC- -5' |
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| 16686 | 5' | -44.1 | NC_004156.1 | + | 221593 | 0.66 | 1 |
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Target: 5'- uGCAGUUGaacuGCUCAaguguuguauaGAGUCGUugCAAAAGa -3' miRNA: 3'- -CGUUAACg---UGGGU-----------UUCAGCA--GUUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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