Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16686 | 5' | -44.1 | NC_004156.1 | + | 99809 | 0.67 | 1 |
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Target: 5'- uGCGAUcacccuuUGaCACUaCAAAGUCGUCAucGGa -3' miRNA: 3'- -CGUUA-------AC-GUGG-GUUUCAGCAGUuuUC- -5' |
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| 16686 | 5' | -44.1 | NC_004156.1 | + | 96179 | 0.66 | 1 |
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Target: 5'- uGCAcccagUGCACCCAGuguGUCcaaucugGUCGAGAc -3' miRNA: 3'- -CGUua---ACGUGGGUUu--CAG-------CAGUUUUc -5' |
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| 16686 | 5' | -44.1 | NC_004156.1 | + | 154627 | 0.66 | 1 |
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Target: 5'- aGCAGUUGCACCUuucuGGUgG-CAGc-- -3' miRNA: 3'- -CGUUAACGUGGGuu--UCAgCaGUUuuc -5' |
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| 16686 | 5' | -44.1 | NC_004156.1 | + | 99348 | 0.67 | 1 |
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Target: 5'- uGCA---GCGCUCAcgAGGUCGUCAu--- -3' miRNA: 3'- -CGUuaaCGUGGGU--UUCAGCAGUuuuc -5' |
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| 16686 | 5' | -44.1 | NC_004156.1 | + | 10913 | 0.67 | 1 |
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Target: 5'- aGCAAUUGCAUuuGcaguuGUUGUCAuuGGu -3' miRNA: 3'- -CGUUAACGUGggUuu---CAGCAGUuuUC- -5' |
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| 16686 | 5' | -44.1 | NC_004156.1 | + | 221593 | 0.66 | 1 |
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Target: 5'- uGCAGUUGaacuGCUCAaguguuguauaGAGUCGUugCAAAAGa -3' miRNA: 3'- -CGUUAACg---UGGGU-----------UUCAGCA--GUUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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