Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 16686 | 5' | -44.1 | NC_004156.1 | + | 62615 | 0.71 | 0.999932 |
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Target: 5'- gGCGAUgagGCAUCCuuGGUCGgCAGAc- -3' miRNA: 3'- -CGUUAa--CGUGGGuuUCAGCaGUUUuc -5' |
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| 16686 | 5' | -44.1 | NC_004156.1 | + | 46538 | 0.71 | 0.999872 |
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Target: 5'- uGCGGUUGCACgugcacaaaauuggCCuu-GUCGUCAGAGu -3' miRNA: 3'- -CGUUAACGUG--------------GGuuuCAGCAGUUUUc -5' |
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| 16686 | 5' | -44.1 | NC_004156.1 | + | 140146 | 0.73 | 0.999487 |
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Target: 5'- ----aUGCACCCAAGGUgGcCAAAGa -3' miRNA: 3'- cguuaACGUGGGUUUCAgCaGUUUUc -5' |
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| 16686 | 5' | -44.1 | NC_004156.1 | + | 61399 | 0.73 | 0.999206 |
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Target: 5'- cGUAAaagGCGCCCuuuGUCGUCAAcAGa -3' miRNA: 3'- -CGUUaa-CGUGGGuuuCAGCAGUUuUC- -5' |
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| 16686 | 5' | -44.1 | NC_004156.1 | + | 144387 | 0.73 | 0.998804 |
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Target: 5'- aGUAAUUGCAgCCAuuuGUCGUaCAAGAu -3' miRNA: 3'- -CGUUAACGUgGGUuu-CAGCA-GUUUUc -5' |
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| 16686 | 5' | -44.1 | NC_004156.1 | + | 144166 | 1.13 | 0.037143 |
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Target: 5'- aGCAAUUGCACCCAAAGUCGUCAAAAGu -3' miRNA: 3'- -CGUUAACGUGGGUUUCAGCAGUUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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