Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16686 | 5' | -44.1 | NC_004156.1 | + | 84415 | 0.68 | 0.999999 |
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Target: 5'- ----cUGCACUCAAGGcUCcUCAGAAGa -3' miRNA: 3'- cguuaACGUGGGUUUC-AGcAGUUUUC- -5' |
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| 16686 | 5' | -44.1 | NC_004156.1 | + | 173786 | 0.68 | 0.999997 |
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Target: 5'- uGCAAUugUGCACCC-AGGUCuaggcacuuGUCAucGGa -3' miRNA: 3'- -CGUUA--ACGUGGGuUUCAG---------CAGUuuUC- -5' |
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| 16686 | 5' | -44.1 | NC_004156.1 | + | 98086 | 0.69 | 0.999993 |
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Target: 5'- -gAGUUGgACUCAGAGUUGgccUCAGAAGu -3' miRNA: 3'- cgUUAACgUGGGUUUCAGC---AGUUUUC- -5' |
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| 16686 | 5' | -44.1 | NC_004156.1 | + | 968 | 0.69 | 0.99999 |
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Target: 5'- -aAAUUGUACCCAuAGcCGUUggGAu -3' miRNA: 3'- cgUUAACGUGGGUuUCaGCAGuuUUc -5' |
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| 16686 | 5' | -44.1 | NC_004156.1 | + | 144387 | 0.73 | 0.998804 |
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Target: 5'- aGUAAUUGCAgCCAuuuGUCGUaCAAGAu -3' miRNA: 3'- -CGUUAACGUgGGUuu-CAGCA-GUUUUc -5' |
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| 16686 | 5' | -44.1 | NC_004156.1 | + | 221593 | 0.66 | 1 |
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Target: 5'- uGCAGUUGaacuGCUCAaguguuguauaGAGUCGUugCAAAAGa -3' miRNA: 3'- -CGUUAACg---UGGGU-----------UUCAGCA--GUUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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