Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16687 | 5' | -53.2 | NC_004156.1 | + | 193608 | 0.72 | 0.823223 |
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Target: 5'- aUCAUGCACAgUACUACGCugcgauCUUugCGUg -3' miRNA: 3'- -AGUACGUGUgGUGAUGUG------GAGugGCA- -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 201963 | 0.68 | 0.951199 |
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Target: 5'- gUCGUGCAagACCACcgACACCggagCGCUGc -3' miRNA: 3'- -AGUACGUg-UGGUGa-UGUGGa---GUGGCa -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 205353 | 0.68 | 0.962462 |
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Target: 5'- uUCGUGCcucCAUCugUGCACCcgugUACCGa -3' miRNA: 3'- -AGUACGu--GUGGugAUGUGGa---GUGGCa -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 208201 | 0.66 | 0.989753 |
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Target: 5'- -aAUGCACACCGCUAgagGCCUacauuaUAUCGUc -3' miRNA: 3'- agUACGUGUGGUGAUg--UGGA------GUGGCA- -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 213399 | 0.67 | 0.97793 |
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Target: 5'- -gAUGCACACUcugaacgacaccgguACUcGCACUUUACCGa -3' miRNA: 3'- agUACGUGUGG---------------UGA-UGUGGAGUGGCa -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 213872 | 0.66 | 0.985221 |
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Target: 5'- -uGUGCACaAUgACUGCACCguauCCGUa -3' miRNA: 3'- agUACGUG-UGgUGAUGUGGagu-GGCA- -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 215276 | 0.71 | 0.863444 |
|
Target: 5'- cUC-UGCACAaaaaCACUACAUC-CACCGUu -3' miRNA: 3'- -AGuACGUGUg---GUGAUGUGGaGUGGCA- -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 218335 | 0.66 | 0.988383 |
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Target: 5'- aCAUGCACucGCCGCUACggaucgaaGCCagGCCc- -3' miRNA: 3'- aGUACGUG--UGGUGAUG--------UGGagUGGca -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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