Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 16687 | 5' | -53.2 | NC_004156.1 | + | 111838 | 0.68 | 0.965777 |
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Target: 5'- -uGUGCACACUGCUACugCaagugguacugUCugCGg -3' miRNA: 3'- agUACGUGUGGUGAUGugG-----------AGugGCa -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 124607 | 0.68 | 0.951199 |
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Target: 5'- -gGUGCAaauguCGCCGCUAC-CC-CACCGa -3' miRNA: 3'- agUACGU-----GUGGUGAUGuGGaGUGGCa -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 201963 | 0.68 | 0.951199 |
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Target: 5'- gUCGUGCAagACCACcgACACCggagCGCUGc -3' miRNA: 3'- -AGUACGUg-UGGUGa-UGUGGa---GUGGCa -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 112903 | 0.69 | 0.942549 |
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Target: 5'- ---aGCGgGCCACUACAUCU-GCCGa -3' miRNA: 3'- aguaCGUgUGGUGAUGUGGAgUGGCa -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 99224 | 0.71 | 0.89126 |
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Target: 5'- cCAUGCACugCACUcaaccauACACUgaaUCACCa- -3' miRNA: 3'- aGUACGUGugGUGA-------UGUGG---AGUGGca -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 151295 | 0.72 | 0.839883 |
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Target: 5'- aUCGuUGCGCACUcggacCACCUCACCGUu -3' miRNA: 3'- -AGU-ACGUGUGGugau-GUGGAGUGGCA- -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 193608 | 0.72 | 0.823223 |
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Target: 5'- aUCAUGCACAgUACUACGCugcgauCUUugCGUg -3' miRNA: 3'- -AGUACGUGUgGUGAUGUG------GAGugGCA- -5' |
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| 16687 | 5' | -53.2 | NC_004156.1 | + | 86492 | 0.66 | 0.989753 |
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Target: 5'- uUCAgGCGuCACCACUGgaaACgUUACCGUc -3' miRNA: 3'- -AGUaCGU-GUGGUGAUg--UGgAGUGGCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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