Results 1 - 20 of 50 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
16998 | 3' | -53.6 | NC_004333.2 | + | 13749 | 0.66 | 0.805662 |
Target: 5'- uCGAGcGCGuaggacGC-CGCGcGCcGCUCGCg -3' miRNA: 3'- -GCUC-CGCua----CGaGCGCaUGuUGAGCG- -5' |
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16998 | 3' | -53.6 | NC_004333.2 | + | 32394 | 0.66 | 0.805662 |
Target: 5'- uCGGGGUucucGGUGUcggcccgcgccUCGC--GCAGCUCGCu -3' miRNA: 3'- -GCUCCG----CUACG-----------AGCGcaUGUUGAGCG- -5' |
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16998 | 3' | -53.6 | NC_004333.2 | + | 39110 | 0.66 | 0.795911 |
Target: 5'- gCGAGGCGcucacGCagaUCgGCGUcgACGugUCGCa -3' miRNA: 3'- -GCUCCGCua---CG---AG-CGCA--UGUugAGCG- -5' |
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16998 | 3' | -53.6 | NC_004333.2 | + | 19510 | 0.66 | 0.795911 |
Target: 5'- --uGGCGgcGCUCGUGgccguauggGCAAUcguUCGCa -3' miRNA: 3'- gcuCCGCuaCGAGCGCa--------UGUUG---AGCG- -5' |
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16998 | 3' | -53.6 | NC_004333.2 | + | 7425 | 0.66 | 0.785985 |
Target: 5'- ---cGCGAUGCgcucgaucagcaUCGCGUucaggaGCGACUUGCc -3' miRNA: 3'- gcucCGCUACG------------AGCGCA------UGUUGAGCG- -5' |
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16998 | 3' | -53.6 | NC_004333.2 | + | 35408 | 0.66 | 0.785985 |
Target: 5'- gCGaAGGCGAgaUUCGCGUcugguACGACUaCGUg -3' miRNA: 3'- -GC-UCCGCUacGAGCGCA-----UGUUGA-GCG- -5' |
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16998 | 3' | -53.6 | NC_004333.2 | + | 40008 | 0.66 | 0.785985 |
Target: 5'- uGAGuacGCGAUGgaCGCGgcCGACcugUCGCc -3' miRNA: 3'- gCUC---CGCUACgaGCGCauGUUG---AGCG- -5' |
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16998 | 3' | -53.6 | NC_004333.2 | + | 32205 | 0.66 | 0.785985 |
Target: 5'- aCGAGcGCGAUGagcacgUCGCcgagcgucUugAGCUCGCg -3' miRNA: 3'- -GCUC-CGCUACg-----AGCGc-------AugUUGAGCG- -5' |
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16998 | 3' | -53.6 | NC_004333.2 | + | 36052 | 0.66 | 0.775894 |
Target: 5'- aCGAcGGCGA-GCUCGU--GCcGCUCGg -3' miRNA: 3'- -GCU-CCGCUaCGAGCGcaUGuUGAGCg -5' |
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16998 | 3' | -53.6 | NC_004333.2 | + | 4607 | 0.66 | 0.775894 |
Target: 5'- uCGAGGCGA-GCgCGCGacacgccauuaaUGCGAUcaggCGCg -3' miRNA: 3'- -GCUCCGCUaCGaGCGC------------AUGUUGa---GCG- -5' |
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16998 | 3' | -53.6 | NC_004333.2 | + | 44391 | 0.66 | 0.765651 |
Target: 5'- --cGGCGgcGCgugCGCGgUACAacggccugccggGCUCGCu -3' miRNA: 3'- gcuCCGCuaCGa--GCGC-AUGU------------UGAGCG- -5' |
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16998 | 3' | -53.6 | NC_004333.2 | + | 18808 | 0.66 | 0.764618 |
Target: 5'- -cGGGCGAcgagccGCUugaagugCGCGUGCGACagCGCa -3' miRNA: 3'- gcUCCGCUa-----CGA-------GCGCAUGUUGa-GCG- -5' |
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16998 | 3' | -53.6 | NC_004333.2 | + | 37322 | 0.66 | 0.755266 |
Target: 5'- uCGcGGCGGUGCUCGaCGcGCucgaaucuGCgUCGCc -3' miRNA: 3'- -GCuCCGCUACGAGC-GCaUGu-------UG-AGCG- -5' |
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16998 | 3' | -53.6 | NC_004333.2 | + | 45101 | 0.66 | 0.755266 |
Target: 5'- gCGAGGCGAUaCUCGaCGccugucugcGCGGCgaUCGCg -3' miRNA: 3'- -GCUCCGCUAcGAGC-GCa--------UGUUG--AGCG- -5' |
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16998 | 3' | -53.6 | NC_004333.2 | + | 27544 | 0.66 | 0.755266 |
Target: 5'- cCGuuGCGAcGCUCGCGUcgaauuggccCGACUgCGCg -3' miRNA: 3'- -GCucCGCUaCGAGCGCAu---------GUUGA-GCG- -5' |
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16998 | 3' | -53.6 | NC_004333.2 | + | 16160 | 0.67 | 0.744752 |
Target: 5'- uGGGGCGA-GUUCGaugucaaGUACGAgUaCGCg -3' miRNA: 3'- gCUCCGCUaCGAGCg------CAUGUUgA-GCG- -5' |
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16998 | 3' | -53.6 | NC_004333.2 | + | 24592 | 0.67 | 0.744752 |
Target: 5'- gGuGGaUGAUGCUCgGCG-GCGGCUuCGCu -3' miRNA: 3'- gCuCC-GCUACGAG-CGCaUGUUGA-GCG- -5' |
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16998 | 3' | -53.6 | NC_004333.2 | + | 21629 | 0.67 | 0.73412 |
Target: 5'- aGAccGGCGcggGCUC-CGUGCAGUUCGCc -3' miRNA: 3'- gCU--CCGCua-CGAGcGCAUGUUGAGCG- -5' |
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16998 | 3' | -53.6 | NC_004333.2 | + | 11855 | 0.67 | 0.73412 |
Target: 5'- cCGuAGGCGAUGCgaCGCccgGCGACUacucgggcggCGCg -3' miRNA: 3'- -GC-UCCGCUACGa-GCGca-UGUUGA----------GCG- -5' |
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16998 | 3' | -53.6 | NC_004333.2 | + | 47498 | 0.67 | 0.73412 |
Target: 5'- aCGAGGCGccgGCcggCGCGaccagUACAuugaaugcACUCGCc -3' miRNA: 3'- -GCUCCGCua-CGa--GCGC-----AUGU--------UGAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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