Results 1 - 20 of 36 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17000 | 3' | -54 | NC_004333.2 | + | 34375 | 0.66 | 0.792049 |
Target: 5'- gCGUCUCGCGcGUCaGUucGAagUGGACGUGAg -3' miRNA: 3'- -GCAGAGUGC-CAG-CA--CU--GCUUGCGCUg -5' |
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17000 | 3' | -54 | NC_004333.2 | + | 20212 | 0.66 | 0.792049 |
Target: 5'- gCGgCUCGCGcuuUCGUGGCGGcgaucgacCGCGACu -3' miRNA: 3'- -GCaGAGUGCc--AGCACUGCUu-------GCGCUG- -5' |
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17000 | 3' | -54 | NC_004333.2 | + | 39989 | 0.66 | 0.782171 |
Target: 5'- aCGUCUCgACGccgcucacugaGUaCGcGAUGGACGCGGCc -3' miRNA: 3'- -GCAGAG-UGC-----------CA-GCaCUGCUUGCGCUG- -5' |
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17000 | 3' | -54 | NC_004333.2 | + | 13350 | 0.66 | 0.782171 |
Target: 5'- -uUCggCACGGU-GU-ACGGGCGCGGCa -3' miRNA: 3'- gcAGa-GUGCCAgCAcUGCUUGCGCUG- -5' |
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17000 | 3' | -54 | NC_004333.2 | + | 9261 | 0.66 | 0.781174 |
Target: 5'- uCGUCUCGugcuuguUGGcCGUGcCGAucaGCGCGAa -3' miRNA: 3'- -GCAGAGU-------GCCaGCACuGCU---UGCGCUg -5' |
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17000 | 3' | -54 | NC_004333.2 | + | 6620 | 0.66 | 0.772136 |
Target: 5'- gCGUUgaagC-CGGUCGUGAgcauCGCGACg -3' miRNA: 3'- -GCAGa---GuGCCAGCACUgcuuGCGCUG- -5' |
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17000 | 3' | -54 | NC_004333.2 | + | 40853 | 0.66 | 0.772136 |
Target: 5'- gCGcCUUcUGGUCGccGGCGAuggucGCGCGACu -3' miRNA: 3'- -GCaGAGuGCCAGCa-CUGCU-----UGCGCUG- -5' |
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17000 | 3' | -54 | NC_004333.2 | + | 32097 | 0.66 | 0.772136 |
Target: 5'- gCGUCggCGCGuUCaGUGACGGcaguuccgACGCGAUa -3' miRNA: 3'- -GCAGa-GUGCcAG-CACUGCU--------UGCGCUG- -5' |
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17000 | 3' | -54 | NC_004333.2 | + | 30684 | 0.66 | 0.761956 |
Target: 5'- gGUCggUGCGGUCGaguGCGAgcagcgugaGCGCGGCg -3' miRNA: 3'- gCAGa-GUGCCAGCac-UGCU---------UGCGCUG- -5' |
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17000 | 3' | -54 | NC_004333.2 | + | 17442 | 0.66 | 0.761956 |
Target: 5'- aCGUCgcccgggCAcCGGaUGUGGCGcAACGCGAa -3' miRNA: 3'- -GCAGa------GU-GCCaGCACUGC-UUGCGCUg -5' |
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17000 | 3' | -54 | NC_004333.2 | + | 33164 | 0.67 | 0.751643 |
Target: 5'- aGUUUCGCGG-CGUcGGCGucgGCGGCc -3' miRNA: 3'- gCAGAGUGCCaGCA-CUGCuugCGCUG- -5' |
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17000 | 3' | -54 | NC_004333.2 | + | 15607 | 0.67 | 0.751643 |
Target: 5'- uCGUCggUCACGGUUucGGCGAACgucgugccguugGCGACc -3' miRNA: 3'- -GCAG--AGUGCCAGcaCUGCUUG------------CGCUG- -5' |
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17000 | 3' | -54 | NC_004333.2 | + | 1024 | 0.67 | 0.720015 |
Target: 5'- gCGUCagAUGcGcaUCGggGACGAACGCGAUg -3' miRNA: 3'- -GCAGagUGC-C--AGCa-CUGCUUGCGCUG- -5' |
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17000 | 3' | -54 | NC_004333.2 | + | 19560 | 0.67 | 0.713584 |
Target: 5'- gGUCgCACGGUCGgcGCGGcacaaggggaaucgcGCGCGAUc -3' miRNA: 3'- gCAGaGUGCCAGCacUGCU---------------UGCGCUG- -5' |
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17000 | 3' | -54 | NC_004333.2 | + | 28955 | 0.67 | 0.709281 |
Target: 5'- gCGUUguucaCGCGGUCGaacgcGGCGAucaguuGCGCGGCc -3' miRNA: 3'- -GCAGa----GUGCCAGCa----CUGCU------UGCGCUG- -5' |
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17000 | 3' | -54 | NC_004333.2 | + | 31909 | 0.67 | 0.709281 |
Target: 5'- aGUCggagC-CGGUCuUGACGAGUGUGACu -3' miRNA: 3'- gCAGa---GuGCCAGcACUGCUUGCGCUG- -5' |
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17000 | 3' | -54 | NC_004333.2 | + | 22885 | 0.67 | 0.698472 |
Target: 5'- uCGUaCUgGCGcUCGUGACGAgcagaaGCGCGGu -3' miRNA: 3'- -GCA-GAgUGCcAGCACUGCU------UGCGCUg -5' |
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17000 | 3' | -54 | NC_004333.2 | + | 40521 | 0.68 | 0.67667 |
Target: 5'- aGUCgaaGCGGUCGUGaACGAAgucguCGCG-Cu -3' miRNA: 3'- gCAGag-UGCCAGCAC-UGCUU-----GCGCuG- -5' |
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17000 | 3' | -54 | NC_004333.2 | + | 33055 | 0.68 | 0.6657 |
Target: 5'- gCGUa--GCGGUCGgccACGAGCGCuGGCa -3' miRNA: 3'- -GCAgagUGCCAGCac-UGCUUGCG-CUG- -5' |
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17000 | 3' | -54 | NC_004333.2 | + | 35447 | 0.68 | 0.654698 |
Target: 5'- uGUUUaguGCGGUCGUGACcgucAACGuCGGCa -3' miRNA: 3'- gCAGAg--UGCCAGCACUGc---UUGC-GCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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