Results 21 - 27 of 27 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 17000 | 5' | -60.3 | NC_004333.2 | + | 9276 | 0.68 | 0.333996 |
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Target: 5'- uGGCCGUgccGAUCagcgcgaagCGGCgcgGgugcgcgGACGGGCg -3' miRNA: 3'- gCCGGCG---CUAGa--------GCCGa--Ca------CUGCCCG- -5' |
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| 17000 | 5' | -60.3 | NC_004333.2 | + | 8982 | 0.69 | 0.296494 |
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Target: 5'- gGGCCGUGcgCggCGGCgaccGACGcGGCg -3' miRNA: 3'- gCCGGCGCuaGa-GCCGaca-CUGC-CCG- -5' |
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| 17000 | 5' | -60.3 | NC_004333.2 | + | 5495 | 0.69 | 0.303725 |
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Target: 5'- uCGGCCGgcguggagaCGAUCggcGCUGUuGCGGGCu -3' miRNA: 3'- -GCCGGC---------GCUAGagcCGACAcUGCCCG- -5' |
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| 17000 | 5' | -60.3 | NC_004333.2 | + | 5038 | 0.66 | 0.437472 |
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Target: 5'- gCGGCCagGCGG-C-CGGCUGUuggccgacgucGGCGcGGCg -3' miRNA: 3'- -GCCGG--CGCUaGaGCCGACA-----------CUGC-CCG- -5' |
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| 17000 | 5' | -60.3 | NC_004333.2 | + | 3936 | 0.67 | 0.374855 |
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Target: 5'- uGGCCGaCGAUCU--GCUucggGACGGGUu -3' miRNA: 3'- gCCGGC-GCUAGAgcCGAca--CUGCCCG- -5' |
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| 17000 | 5' | -60.3 | NC_004333.2 | + | 2176 | 0.7 | 0.255914 |
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Target: 5'- aGGCCGUGccagccCUCGGCgccgaGUGGCaGGCc -3' miRNA: 3'- gCCGGCGCua----GAGCCGa----CACUGcCCG- -5' |
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| 17000 | 5' | -60.3 | NC_004333.2 | + | 849 | 0.66 | 0.456438 |
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Target: 5'- -aGCUuCGAUCUCGGCUuucGUG-CGGcGCu -3' miRNA: 3'- gcCGGcGCUAGAGCCGA---CACuGCC-CG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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