Results 1 - 20 of 99 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17003 | 3' | -56.9 | NC_004333.2 | + | 3391 | 0.66 | 0.592358 |
Target: 5'- gGCGUCGCCGuCGUCGcgggagcuucggugaAGAacaGCUGCg- -3' miRNA: 3'- -UGCGGCGGUuGUAGC---------------UCUg--CGACGag -5' |
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17003 | 3' | -56.9 | NC_004333.2 | + | 25537 | 0.66 | 0.592358 |
Target: 5'- cGCGCCGCUcgaggucagcgggucGACAUUGGcGAUcaGCUGCg- -3' miRNA: 3'- -UGCGGCGG---------------UUGUAGCU-CUG--CGACGag -5' |
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17003 | 3' | -56.9 | NC_004333.2 | + | 20509 | 0.66 | 0.587953 |
Target: 5'- cCGCUGCCGGCcgcCGccGCGCcGCUCg -3' miRNA: 3'- uGCGGCGGUUGua-GCucUGCGaCGAG- -5' |
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17003 | 3' | -56.9 | NC_004333.2 | + | 5151 | 0.66 | 0.587953 |
Target: 5'- -aGCCGCCcguguUGUCGAGcaGCGCaGCUUg -3' miRNA: 3'- ugCGGCGGuu---GUAGCUC--UGCGaCGAG- -5' |
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17003 | 3' | -56.9 | NC_004333.2 | + | 17477 | 0.66 | 0.587953 |
Target: 5'- -gGCCGCC-GCGcUC---ACGCUGCUCg -3' miRNA: 3'- ugCGGCGGuUGU-AGcucUGCGACGAG- -5' |
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17003 | 3' | -56.9 | NC_004333.2 | + | 17010 | 0.66 | 0.587953 |
Target: 5'- uACGCCGUCGccGCGcucgacUCGuGcACGCUGCa- -3' miRNA: 3'- -UGCGGCGGU--UGU------AGCuC-UGCGACGag -5' |
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17003 | 3' | -56.9 | NC_004333.2 | + | 17368 | 0.66 | 0.584654 |
Target: 5'- -aGUCGCUgugggagGACGUCGAGcagcgcaucgaGCUGCUCg -3' miRNA: 3'- ugCGGCGG-------UUGUAGCUCug---------CGACGAG- -5' |
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17003 | 3' | -56.9 | NC_004333.2 | + | 36268 | 0.66 | 0.576969 |
Target: 5'- cGCGCCGCUGcGCAUgcCGGGcCGCgacgacaaggUGCUCg -3' miRNA: 3'- -UGCGGCGGU-UGUA--GCUCuGCG----------ACGAG- -5' |
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17003 | 3' | -56.9 | NC_004333.2 | + | 20557 | 0.66 | 0.576969 |
Target: 5'- cGCGCCGCUGAuCGUCGGcGCGUcgGgUCg -3' miRNA: 3'- -UGCGGCGGUU-GUAGCUcUGCGa-CgAG- -5' |
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17003 | 3' | -56.9 | NC_004333.2 | + | 28465 | 0.66 | 0.576969 |
Target: 5'- cACGCCaCCAGCGcCGAGcACGCgaGCa- -3' miRNA: 3'- -UGCGGcGGUUGUaGCUC-UGCGa-CGag -5' |
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17003 | 3' | -56.9 | NC_004333.2 | + | 15900 | 0.66 | 0.575872 |
Target: 5'- uCGCCGCCGACccgcgccAUCcGGAaggcuuucggcCGCUGCUg -3' miRNA: 3'- uGCGGCGGUUG-------UAGcUCU-----------GCGACGAg -5' |
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17003 | 3' | -56.9 | NC_004333.2 | + | 17079 | 0.66 | 0.572587 |
Target: 5'- cGCGCCGCUGACGggCcAGGCGCcgaaccugcaggcGCUCg -3' miRNA: 3'- -UGCGGCGGUUGUa-GcUCUGCGa------------CGAG- -5' |
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17003 | 3' | -56.9 | NC_004333.2 | + | 6631 | 0.66 | 0.566029 |
Target: 5'- -gGUCGUgAGCAUCGcGACGCUGaCg- -3' miRNA: 3'- ugCGGCGgUUGUAGCuCUGCGAC-Gag -5' |
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17003 | 3' | -56.9 | NC_004333.2 | + | 34298 | 0.66 | 0.566029 |
Target: 5'- gUGCCgGCCGACuUCGcgcGGCGCggcgcGCUCa -3' miRNA: 3'- uGCGG-CGGUUGuAGCu--CUGCGa----CGAG- -5' |
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17003 | 3' | -56.9 | NC_004333.2 | + | 38929 | 0.66 | 0.566029 |
Target: 5'- uAUGCCGCacGCggCGAGGCGCccccGUUCg -3' miRNA: 3'- -UGCGGCGguUGuaGCUCUGCGa---CGAG- -5' |
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17003 | 3' | -56.9 | NC_004333.2 | + | 15291 | 0.66 | 0.566029 |
Target: 5'- gGCGCgGUCAGCAUCagcaccGGGCGCUuGCcgUCg -3' miRNA: 3'- -UGCGgCGGUUGUAGc-----UCUGCGA-CG--AG- -5' |
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17003 | 3' | -56.9 | NC_004333.2 | + | 273 | 0.66 | 0.555145 |
Target: 5'- cACGCCGCCgAGCA-CGGucacggucGACGuCUGCg- -3' miRNA: 3'- -UGCGGCGG-UUGUaGCU--------CUGC-GACGag -5' |
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17003 | 3' | -56.9 | NC_004333.2 | + | 16856 | 0.66 | 0.555145 |
Target: 5'- uCGCgGCCAagGCgAUCGAcgGGCGaCUGUUCg -3' miRNA: 3'- uGCGgCGGU--UG-UAGCU--CUGC-GACGAG- -5' |
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17003 | 3' | -56.9 | NC_004333.2 | + | 36848 | 0.66 | 0.544322 |
Target: 5'- cGCGCCGCCuuCAUCGc-GCGCgaccagUGCa- -3' miRNA: 3'- -UGCGGCGGuuGUAGCucUGCG------ACGag -5' |
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17003 | 3' | -56.9 | NC_004333.2 | + | 8195 | 0.66 | 0.544322 |
Target: 5'- cGCGCCagucaaaaacGCCAGCAccucauuagUCGGGcacucuucgaGCGCuUGCUCg -3' miRNA: 3'- -UGCGG----------CGGUUGU---------AGCUC----------UGCG-ACGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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