Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 17153 | 3' | -60.9 | NC_004333.2 | + | 25413 | 0.66 | 0.374855 |
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Target: 5'- gCGCCUGcagcGUGCCGgcggcGGCCGuCACGc-- -3' miRNA: 3'- -GCGGGC----CACGGCa----CCGGCuGUGCaag -5' |
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| 17153 | 3' | -60.9 | NC_004333.2 | + | 47609 | 0.66 | 0.374855 |
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Target: 5'- aGCUgCGcGUcGCCGagaUGGgCGGCACGUUCg -3' miRNA: 3'- gCGG-GC-CA-CGGC---ACCgGCUGUGCAAG- -5' |
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| 17153 | 3' | -60.9 | NC_004333.2 | + | 18537 | 0.66 | 0.383423 |
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Target: 5'- aCGCcuCCGGUGCCG-GGuuG-CGCGa-- -3' miRNA: 3'- -GCG--GGCCACGGCaCCggCuGUGCaag -5' |
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| 17153 | 3' | -60.9 | NC_004333.2 | + | 2291 | 0.66 | 0.400944 |
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Target: 5'- gGCaaguCGuGUaCCGUGGCCGGCACGc-- -3' miRNA: 3'- gCGg---GC-CAcGGCACCGGCUGUGCaag -5' |
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| 17153 | 3' | -60.9 | NC_004333.2 | + | 2749 | 0.66 | 0.408993 |
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Target: 5'- aGCCgagaucgCGGccGCCGU-GCCGGuCGCGUUCg -3' miRNA: 3'- gCGG-------GCCa-CGGCAcCGGCU-GUGCAAG- -5' |
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| 17153 | 3' | -60.9 | NC_004333.2 | + | 32839 | 0.66 | 0.408993 |
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Target: 5'- uGCaaGGUGCCGcgcuuaUGGCCGGCggcagcgaccugcGCGUg- -3' miRNA: 3'- gCGggCCACGGC------ACCGGCUG-------------UGCAag -5' |
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| 17153 | 3' | -60.9 | NC_004333.2 | + | 5533 | 0.66 | 0.409893 |
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Target: 5'- gGUCa--UGCCGUGGCCGGCgACGg-- -3' miRNA: 3'- gCGGgccACGGCACCGGCUG-UGCaag -5' |
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| 17153 | 3' | -60.9 | NC_004333.2 | + | 38626 | 0.66 | 0.418966 |
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Target: 5'- uGCCCguGGUGcCCGUucugaucggcccGGgCGGCACGggCa -3' miRNA: 3'- gCGGG--CCAC-GGCA------------CCgGCUGUGCaaG- -5' |
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| 17153 | 3' | -60.9 | NC_004333.2 | + | 37752 | 0.66 | 0.42816 |
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Target: 5'- aCGCgCCGG-GCC--GGCCGACgaGCGggUCg -3' miRNA: 3'- -GCG-GGCCaCGGcaCCGGCUG--UGCa-AG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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