Results 1 - 20 of 64 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
17155 | 3' | -50.3 | NC_004333.2 | + | 18438 | 1.1 | 0.002692 |
Target: 5'- gUCGACACCGCGAGCGCGUUCAAAAAGu -3' miRNA: 3'- -AGCUGUGGCGCUCGCGCAAGUUUUUC- -5' |
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17155 | 3' | -50.3 | NC_004333.2 | + | 20166 | 0.67 | 0.886879 |
Target: 5'- cUCGGcCGCCGgcuCGGGCGCacaCAAGAAGg -3' miRNA: 3'- -AGCU-GUGGC---GCUCGCGcaaGUUUUUC- -5' |
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17155 | 3' | -50.3 | NC_004333.2 | + | 46862 | 0.67 | 0.894414 |
Target: 5'- aUCGACGCCGU--GCGC--UCGAAAGa -3' miRNA: 3'- -AGCUGUGGCGcuCGCGcaAGUUUUUc -5' |
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17155 | 3' | -50.3 | NC_004333.2 | + | 37729 | 0.66 | 0.93347 |
Target: 5'- gUCGGCaagcuGCCGCGccuGCGCGagaUCGAcGAGc -3' miRNA: 3'- -AGCUG-----UGGCGCu--CGCGCa--AGUUuUUC- -5' |
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17155 | 3' | -50.3 | NC_004333.2 | + | 30232 | 0.72 | 0.619699 |
Target: 5'- aUCGACgcGCCGCGcgcgaucAGUGCGUUCAu---- -3' miRNA: 3'- -AGCUG--UGGCGC-------UCGCGCAAGUuuuuc -5' |
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17155 | 3' | -50.3 | NC_004333.2 | + | 46887 | 0.71 | 0.677999 |
Target: 5'- cCGAgUGCCGCGAGCGCcuguGUUCAu---- -3' miRNA: 3'- aGCU-GUGGCGCUCGCG----CAAGUuuuuc -5' |
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17155 | 3' | -50.3 | NC_004333.2 | + | 46919 | 0.71 | 0.689336 |
Target: 5'- --aGCGCCGCGAGCGCcg-CGAAGc- -3' miRNA: 3'- agcUGUGGCGCUCGCGcaaGUUUUuc -5' |
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17155 | 3' | -50.3 | NC_004333.2 | + | 45287 | 0.7 | 0.733963 |
Target: 5'- aCGACGucCCGaagcuCGAG-GCGUUCGAGAAGg -3' miRNA: 3'- aGCUGU--GGC-----GCUCgCGCAAGUUUUUC- -5' |
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17155 | 3' | -50.3 | NC_004333.2 | + | 30832 | 0.69 | 0.807303 |
Target: 5'- gUCGACACCGCGucguagaaCGCG-UCGAGc-- -3' miRNA: 3'- -AGCUGUGGCGCuc------GCGCaAGUUUuuc -5' |
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17155 | 3' | -50.3 | NC_004333.2 | + | 10866 | 0.67 | 0.861758 |
Target: 5'- cUUGACGCCGCGcgggaagaacgccGGCGCGacagUCAc---- -3' miRNA: 3'- -AGCUGUGGCGC-------------UCGCGCa---AGUuuuuc -5' |
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17155 | 3' | -50.3 | NC_004333.2 | + | 44573 | 0.68 | 0.817082 |
Target: 5'- aUCGACACCGaCGcgauggucGGCGUGUggGAAAAc -3' miRNA: 3'- -AGCUGUGGC-GC--------UCGCGCAagUUUUUc -5' |
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17155 | 3' | -50.3 | NC_004333.2 | + | 43074 | 0.69 | 0.797325 |
Target: 5'- gUGACGCCGCacaucGAGaCGCGaUCAAcGAGu -3' miRNA: 3'- aGCUGUGGCG-----CUC-GCGCaAGUUuUUC- -5' |
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17155 | 3' | -50.3 | NC_004333.2 | + | 34963 | 0.74 | 0.519423 |
Target: 5'- cCGGCcauuGCCGUGAGUGCGUUUugcAGAGg -3' miRNA: 3'- aGCUG----UGGCGCUCGCGCAAGuu-UUUC- -5' |
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17155 | 3' | -50.3 | NC_004333.2 | + | 25216 | 0.68 | 0.826652 |
Target: 5'- gUCGACGcCCGCGAcGCuGcCGUUCGGGu-- -3' miRNA: 3'- -AGCUGU-GGCGCU-CG-C-GCAAGUUUuuc -5' |
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17155 | 3' | -50.3 | NC_004333.2 | + | 13120 | 0.73 | 0.541529 |
Target: 5'- aCGGCAUCGCGAGCuGCG-UCAu---- -3' miRNA: 3'- aGCUGUGGCGCUCG-CGCaAGUuuuuc -5' |
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17155 | 3' | -50.3 | NC_004333.2 | + | 19623 | 0.7 | 0.755664 |
Target: 5'- gCGAgCGCgGCGAGCGCGgcCGAc--- -3' miRNA: 3'- aGCU-GUGgCGCUCGCGCaaGUUuuuc -5' |
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17155 | 3' | -50.3 | NC_004333.2 | + | 44389 | 0.68 | 0.835999 |
Target: 5'- cUCGGCGgCGCGuGCGCGguaCAAc--- -3' miRNA: 3'- -AGCUGUgGCGCuCGCGCaa-GUUuuuc -5' |
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17155 | 3' | -50.3 | NC_004333.2 | + | 43590 | 0.67 | 0.886879 |
Target: 5'- cUCGAa--CGCGAGUGCGcgagCAAGAAu -3' miRNA: 3'- -AGCUgugGCGCUCGCGCaa--GUUUUUc -5' |
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17155 | 3' | -50.3 | NC_004333.2 | + | 6650 | 0.73 | 0.563939 |
Target: 5'- cUCGGCAgCGCGcucGGCGCGcUCGAGcAGg -3' miRNA: 3'- -AGCUGUgGCGC---UCGCGCaAGUUUuUC- -5' |
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17155 | 3' | -50.3 | NC_004333.2 | + | 12633 | 0.71 | 0.689336 |
Target: 5'- gUCGAguUCGCGaAGCGCGUUCc----- -3' miRNA: 3'- -AGCUguGGCGC-UCGCGCAAGuuuuuc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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