Results 1 - 20 of 25 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18002 | 3' | -50.9 | NC_004665.1 | + | 35495 | 0.66 | 0.859388 |
Target: 5'- cGUAUCCACG--GCCuu-CCAgcUCGCGGu -3' miRNA: 3'- -CGUAGGUGUguUGGucuGGU--AGUGCC- -5' |
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18002 | 3' | -50.9 | NC_004665.1 | + | 22463 | 0.66 | 0.859388 |
Target: 5'- uCAUCUAuCAgGuCCAGACCuUCACGa -3' miRNA: 3'- cGUAGGU-GUgUuGGUCUGGuAGUGCc -5' |
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18002 | 3' | -50.9 | NC_004665.1 | + | 12996 | 0.66 | 0.850586 |
Target: 5'- aGCGUCCAUGCAGCagcgugcuCCAU-ACGGa -3' miRNA: 3'- -CGUAGGUGUGUUGgucu----GGUAgUGCC- -5' |
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18002 | 3' | -50.9 | NC_004665.1 | + | 14562 | 0.66 | 0.84153 |
Target: 5'- gGUGUCgGuCGCAACCAcGAUgGUCAuCGGg -3' miRNA: 3'- -CGUAGgU-GUGUUGGU-CUGgUAGU-GCC- -5' |
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18002 | 3' | -50.9 | NC_004665.1 | + | 3326 | 0.66 | 0.84153 |
Target: 5'- cGCAUUCAaGCGACCGuGACUGUC-CGa -3' miRNA: 3'- -CGUAGGUgUGUUGGU-CUGGUAGuGCc -5' |
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18002 | 3' | -50.9 | NC_004665.1 | + | 33278 | 0.66 | 0.840611 |
Target: 5'- uGCAUCCAcCACAACUGGuugucaguguucaGCCAgagCGCa- -3' miRNA: 3'- -CGUAGGU-GUGUUGGUC-------------UGGUa--GUGcc -5' |
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18002 | 3' | -50.9 | NC_004665.1 | + | 1284 | 0.67 | 0.8227 |
Target: 5'- -gGUCC-CGauguuguCCaAGGCCAUCACGGa -3' miRNA: 3'- cgUAGGuGUguu----GG-UCUGGUAGUGCC- -5' |
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18002 | 3' | -50.9 | NC_004665.1 | + | 7074 | 0.67 | 0.802986 |
Target: 5'- aGCAUCCucaGCGAUggacucaaGGACCGUCGCa- -3' miRNA: 3'- -CGUAGGug-UGUUGg-------UCUGGUAGUGcc -5' |
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18002 | 3' | -50.9 | NC_004665.1 | + | 18455 | 0.67 | 0.802986 |
Target: 5'- aGCggUCACACGcucACCcGACCGUUgaACGGc -3' miRNA: 3'- -CGuaGGUGUGU---UGGuCUGGUAG--UGCC- -5' |
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18002 | 3' | -50.9 | NC_004665.1 | + | 21716 | 0.68 | 0.771965 |
Target: 5'- gGUAUCuCGCaACAACCAGA-CAUCcuuCGGu -3' miRNA: 3'- -CGUAG-GUG-UGUUGGUCUgGUAGu--GCC- -5' |
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18002 | 3' | -50.9 | NC_004665.1 | + | 27555 | 0.68 | 0.771965 |
Target: 5'- cGCcaagUCGC-CAACCGGGCC-UCugGGu -3' miRNA: 3'- -CGua--GGUGuGUUGGUCUGGuAGugCC- -5' |
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18002 | 3' | -50.9 | NC_004665.1 | + | 24446 | 0.68 | 0.765577 |
Target: 5'- cGCAUCCGuugucccuuggucaGCGACUGGACCAcauuggucgUCACGa -3' miRNA: 3'- -CGUAGGUg-------------UGUUGGUCUGGU---------AGUGCc -5' |
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18002 | 3' | -50.9 | NC_004665.1 | + | 32772 | 0.68 | 0.728436 |
Target: 5'- gGCcgCCACcauGCAAUCGGGCCugacaGUCAaCGGu -3' miRNA: 3'- -CGuaGGUG---UGUUGGUCUGG-----UAGU-GCC- -5' |
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18002 | 3' | -50.9 | NC_004665.1 | + | 15188 | 0.68 | 0.728436 |
Target: 5'- aGCGUCCACACGucuucgccCCAGuCCAcuucuuccUCACuGGc -3' miRNA: 3'- -CGUAGGUGUGUu-------GGUCuGGU--------AGUG-CC- -5' |
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18002 | 3' | -50.9 | NC_004665.1 | + | 12550 | 0.69 | 0.70598 |
Target: 5'- -gAUCCcguuguggaACACGAUgAGACCAUC-CGGc -3' miRNA: 3'- cgUAGG---------UGUGUUGgUCUGGUAGuGCC- -5' |
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18002 | 3' | -50.9 | NC_004665.1 | + | 6175 | 0.7 | 0.660216 |
Target: 5'- uCGUCCugACugacugccgugaGGCuCAGAUCGUCAUGGa -3' miRNA: 3'- cGUAGGugUG------------UUG-GUCUGGUAGUGCC- -5' |
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18002 | 3' | -50.9 | NC_004665.1 | + | 20540 | 0.7 | 0.660216 |
Target: 5'- aGCuUCgACAuCGACCAGAUCAgcaGCGGc -3' miRNA: 3'- -CGuAGgUGU-GUUGGUCUGGUag-UGCC- -5' |
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18002 | 3' | -50.9 | NC_004665.1 | + | 21293 | 0.7 | 0.660216 |
Target: 5'- cCAgCCACGCGAUUGGACU-UCGCGGu -3' miRNA: 3'- cGUaGGUGUGUUGGUCUGGuAGUGCC- -5' |
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18002 | 3' | -50.9 | NC_004665.1 | + | 4788 | 0.7 | 0.656755 |
Target: 5'- uGCAggUACugGACCAGAgCCGggaagcgcuugcccUCACGGg -3' miRNA: 3'- -CGUagGUGugUUGGUCU-GGU--------------AGUGCC- -5' |
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18002 | 3' | -50.9 | NC_004665.1 | + | 2782 | 0.7 | 0.648671 |
Target: 5'- aGCAgguUCacaGCACGACCAccacGCuCAUCACGGa -3' miRNA: 3'- -CGU---AGg--UGUGUUGGUc---UG-GUAGUGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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