Results 1 - 20 of 35 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18052 | 3' | -63.2 | NC_004680.1 | + | 8638 | 0.66 | 0.412103 |
Target: 5'- gUGGCCUGUucaCGCGCGGCGGcaccaUUGUUc -3' miRNA: 3'- gGCCGGGCGg--GUGCGCCGCU-----AGCAGc -5' |
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18052 | 3' | -63.2 | NC_004680.1 | + | 7369 | 0.66 | 0.39502 |
Target: 5'- cUCGGCCUcauucucagcgGCCUcaucagACGCGGCGAaCGUg- -3' miRNA: 3'- -GGCCGGG-----------CGGG------UGCGCCGCUaGCAgc -5' |
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18052 | 3' | -63.2 | NC_004680.1 | + | 48184 | 0.66 | 0.386652 |
Target: 5'- gCCGGCgcucaugacauCCGCCCACGCGccaaaGCGccCGUa- -3' miRNA: 3'- -GGCCG-----------GGCGGGUGCGC-----CGCuaGCAgc -5' |
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18052 | 3' | -63.2 | NC_004680.1 | + | 35080 | 0.66 | 0.386652 |
Target: 5'- cCCGGCgcaaCGUCCAUGUuguuGGCGGacacUCGUCc -3' miRNA: 3'- -GGCCGg---GCGGGUGCG----CCGCU----AGCAGc -5' |
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18052 | 3' | -63.2 | NC_004680.1 | + | 25409 | 0.66 | 0.378402 |
Target: 5'- gCGGCCCucGUCUACGaGGCGAUgacUGUCc -3' miRNA: 3'- gGCCGGG--CGGGUGCgCCGCUA---GCAGc -5' |
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18052 | 3' | -63.2 | NC_004680.1 | + | 34570 | 0.66 | 0.378402 |
Target: 5'- cCCGGCUg---CACGUgGGCGAUCGUCa -3' miRNA: 3'- -GGCCGGgcggGUGCG-CCGCUAGCAGc -5' |
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18052 | 3' | -63.2 | NC_004680.1 | + | 17238 | 0.66 | 0.37595 |
Target: 5'- gCGGCUgaUCCugGUGGCGGgaccugcggcggguUCGUCGg -3' miRNA: 3'- gGCCGGgcGGGugCGCCGCU--------------AGCAGC- -5' |
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18052 | 3' | -63.2 | NC_004680.1 | + | 52200 | 0.66 | 0.37027 |
Target: 5'- gCCGGCaaGCCgACGagccaggaGGCGGauaCGUCGa -3' miRNA: 3'- -GGCCGggCGGgUGCg-------CCGCUa--GCAGC- -5' |
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18052 | 3' | -63.2 | NC_004680.1 | + | 22225 | 0.67 | 0.362259 |
Target: 5'- aCCGGgagaCUGCgCGCGCGGCG-UCGg-- -3' miRNA: 3'- -GGCCg---GGCGgGUGCGCCGCuAGCagc -5' |
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18052 | 3' | -63.2 | NC_004680.1 | + | 9806 | 0.67 | 0.359089 |
Target: 5'- cCCGGCCCGCCgAcacaccauucgcCGCGaacccauccacaauGCGAgaacccgcagccuUCGUCGc -3' miRNA: 3'- -GGCCGGGCGGgU------------GCGC--------------CGCU-------------AGCAGC- -5' |
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18052 | 3' | -63.2 | NC_004680.1 | + | 27112 | 0.67 | 0.354369 |
Target: 5'- gCCGGCCaaCCugggguugUugGCGGCGcgccuguaGUCGUCGg -3' miRNA: 3'- -GGCCGGgcGG--------GugCGCCGC--------UAGCAGC- -5' |
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18052 | 3' | -63.2 | NC_004680.1 | + | 826 | 0.67 | 0.354369 |
Target: 5'- aCGGCCagccaaacggUGCCCACGCGGauacaGcUCGUa- -3' miRNA: 3'- gGCCGG----------GCGGGUGCGCCg----CuAGCAgc -5' |
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18052 | 3' | -63.2 | NC_004680.1 | + | 21444 | 0.67 | 0.346602 |
Target: 5'- gCCGGCgCCGCCaUACGUGGcCGA-CGg-- -3' miRNA: 3'- -GGCCG-GGCGG-GUGCGCC-GCUaGCagc -5' |
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18052 | 3' | -63.2 | NC_004680.1 | + | 28157 | 0.67 | 0.331435 |
Target: 5'- uCgGGCCUGCauuuCCGCgaccGCGGCGGucaucugcUCGUCGu -3' miRNA: 3'- -GgCCGGGCG----GGUG----CGCCGCU--------AGCAGC- -5' |
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18052 | 3' | -63.2 | NC_004680.1 | + | 44638 | 0.67 | 0.331435 |
Target: 5'- -gGGCgUCGaUCAUGCGGCGuUCGUCGg -3' miRNA: 3'- ggCCG-GGCgGGUGCGCCGCuAGCAGC- -5' |
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18052 | 3' | -63.2 | NC_004680.1 | + | 12412 | 0.68 | 0.316761 |
Target: 5'- cCCGGCgCGgcaCCCgaaGCGGCGGUCGcCc -3' miRNA: 3'- -GGCCGgGC---GGGug-CGCCGCUAGCaGc -5' |
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18052 | 3' | -63.2 | NC_004680.1 | + | 49600 | 0.68 | 0.30961 |
Target: 5'- -aGGCCCGCCCAcCGCcuGCauuccAUCGUCc -3' miRNA: 3'- ggCCGGGCGGGU-GCGc-CGc----UAGCAGc -5' |
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18052 | 3' | -63.2 | NC_004680.1 | + | 30921 | 0.68 | 0.302583 |
Target: 5'- uCgGGCUgGCCUACG-GGUgugauGAUCGUCGu -3' miRNA: 3'- -GgCCGGgCGGGUGCgCCG-----CUAGCAGC- -5' |
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18052 | 3' | -63.2 | NC_004680.1 | + | 43771 | 0.68 | 0.301887 |
Target: 5'- -aGGCCCuGUCCcguguggAUGCGGUGAUCGggUCGu -3' miRNA: 3'- ggCCGGG-CGGG-------UGCGCCGCUAGC--AGC- -5' |
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18052 | 3' | -63.2 | NC_004680.1 | + | 22772 | 0.68 | 0.288227 |
Target: 5'- gUCGGCguCCGCCCcgggagaAgGCGGCcgaGUCGUCGu -3' miRNA: 3'- -GGCCG--GGCGGG-------UgCGCCGc--UAGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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