Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18055 | 3' | -51.2 | NC_004680.1 | + | 20888 | 0.66 | 0.930122 |
Target: 5'- aACG-CGCCGGCAgCGUCGAGgaaguuCGGg- -3' miRNA: 3'- -UGCuGCGGUCGU-GCAGUUUau----GCUga -5' |
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18055 | 3' | -51.2 | NC_004680.1 | + | 34648 | 0.66 | 0.926654 |
Target: 5'- gGCGACGgCacccacaacaugggcGGCGCaGaCGGAUACGACUg -3' miRNA: 3'- -UGCUGCgG---------------UCGUG-CaGUUUAUGCUGA- -5' |
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18055 | 3' | -51.2 | NC_004680.1 | + | 23150 | 0.66 | 0.926654 |
Target: 5'- aGCGGCGCCAGUgugaacacugauccaGuCGcCAAGaGCGGCg -3' miRNA: 3'- -UGCUGCGGUCG---------------U-GCaGUUUaUGCUGa -5' |
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18055 | 3' | -51.2 | NC_004680.1 | + | 51099 | 0.66 | 0.924284 |
Target: 5'- cCGACGagaAGCGCGUCAAGUuccCGcACg -3' miRNA: 3'- uGCUGCgg-UCGUGCAGUUUAu--GC-UGa -5' |
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18055 | 3' | -51.2 | NC_004680.1 | + | 3993 | 0.66 | 0.915625 |
Target: 5'- uACGGCGCUgaccGGCACGgagugccgcUGCGACg -3' miRNA: 3'- -UGCUGCGG----UCGUGCaguuu----AUGCUGa -5' |
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18055 | 3' | -51.2 | NC_004680.1 | + | 56231 | 0.66 | 0.912396 |
Target: 5'- gACGACcCCAGcCGCGUCGAAguucucauccucacCGACa -3' miRNA: 3'- -UGCUGcGGUC-GUGCAGUUUau------------GCUGa -5' |
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18055 | 3' | -51.2 | NC_004680.1 | + | 41443 | 0.66 | 0.911741 |
Target: 5'- -aGACuGUCAGCuucaGCGUguGGUGCGGCg -3' miRNA: 3'- ugCUG-CGGUCG----UGCAguUUAUGCUGa -5' |
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18055 | 3' | -51.2 | NC_004680.1 | + | 25643 | 0.66 | 0.905039 |
Target: 5'- cCGACGCC-GCACc-CAAAcacUGCGACa -3' miRNA: 3'- uGCUGCGGuCGUGcaGUUU---AUGCUGa -5' |
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18055 | 3' | -51.2 | NC_004680.1 | + | 18377 | 0.66 | 0.905039 |
Target: 5'- aACGugcCGCCAGUAgCGUCuuccgugACGACg -3' miRNA: 3'- -UGCu--GCGGUCGU-GCAGuuua---UGCUGa -5' |
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18055 | 3' | -51.2 | NC_004680.1 | + | 43513 | 0.66 | 0.904353 |
Target: 5'- cACGACGCCuccuccgcgaccuAGUGCGUUg---ACGACg -3' miRNA: 3'- -UGCUGCGG-------------UCGUGCAGuuuaUGCUGa -5' |
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18055 | 3' | -51.2 | NC_004680.1 | + | 28340 | 0.66 | 0.897339 |
Target: 5'- gACGGCGCgGGCggugucgGCGUCGGGgcCGAUc -3' miRNA: 3'- -UGCUGCGgUCG-------UGCAGUUUauGCUGa -5' |
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18055 | 3' | -51.2 | NC_004680.1 | + | 57239 | 0.67 | 0.874639 |
Target: 5'- gGCGGCGCCGGgGaauccgugccauuCGUCGAAcACGAg- -3' miRNA: 3'- -UGCUGCGGUCgU-------------GCAGUUUaUGCUga -5' |
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18055 | 3' | -51.2 | NC_004680.1 | + | 41818 | 0.67 | 0.85903 |
Target: 5'- aACGAgGCCGGCGUGUgGG--GCGGCUu -3' miRNA: 3'- -UGCUgCGGUCGUGCAgUUuaUGCUGA- -5' |
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18055 | 3' | -51.2 | NC_004680.1 | + | 41853 | 0.67 | 0.858183 |
Target: 5'- cAUGGCGCUuggacggGGCAgGUCcagcAGAUGCGGCg -3' miRNA: 3'- -UGCUGCGG-------UCGUgCAG----UUUAUGCUGa -5' |
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18055 | 3' | -51.2 | NC_004680.1 | + | 27667 | 0.68 | 0.823314 |
Target: 5'- -aGAUGCCGGgGCGgccauUCAcGUACGGCg -3' miRNA: 3'- ugCUGCGGUCgUGC-----AGUuUAUGCUGa -5' |
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18055 | 3' | -51.2 | NC_004680.1 | + | 28845 | 0.68 | 0.813834 |
Target: 5'- -gGACGCCGGCAUGUUcuucGCGAa- -3' miRNA: 3'- ugCUGCGGUCGUGCAGuuuaUGCUga -5' |
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18055 | 3' | -51.2 | NC_004680.1 | + | 11418 | 0.69 | 0.801212 |
Target: 5'- gGCGGCGCgGGCACGcuucuccgccagUuuguggcgggcaucCAGAUACGACg -3' miRNA: 3'- -UGCUGCGgUCGUGC------------A--------------GUUUAUGCUGa -5' |
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18055 | 3' | -51.2 | NC_004680.1 | + | 4949 | 0.69 | 0.774028 |
Target: 5'- cCGACGUCGGCACGgccg--ACGAUg -3' miRNA: 3'- uGCUGCGGUCGUGCaguuuaUGCUGa -5' |
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18055 | 3' | -51.2 | NC_004680.1 | + | 25339 | 0.69 | 0.763663 |
Target: 5'- gACGAgggcCGCCAGUucgGCGUCAAGcuCGGCg -3' miRNA: 3'- -UGCU----GCGGUCG---UGCAGUUUauGCUGa -5' |
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18055 | 3' | -51.2 | NC_004680.1 | + | 43531 | 0.69 | 0.763663 |
Target: 5'- uGCGACGCCGcCACGuUCGAccaggGCGGCc -3' miRNA: 3'- -UGCUGCGGUcGUGC-AGUUua---UGCUGa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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