Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 18058 | 5' | -55.8 | NC_004680.1 | + | 3082 | 0.68 | 0.560121 |
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Target: 5'- gCCGC-GCCGGGAGcGUUguggGAGUUGCCc -3' miRNA: 3'- -GGCGaCGGCCUUU-CGGa---CUUAGCGGu -5' |
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| 18058 | 5' | -55.8 | NC_004680.1 | + | 5898 | 0.68 | 0.560121 |
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Target: 5'- aCGUUGCUGcGgcGCUUGGAUUGCCGa -3' miRNA: 3'- gGCGACGGCcUuuCGGACUUAGCGGU- -5' |
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| 18058 | 5' | -55.8 | NC_004680.1 | + | 24849 | 0.69 | 0.517731 |
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Target: 5'- gCCGCUGcCCGGAGAuCCcgaggUGGAUgUGCCAc -3' miRNA: 3'- -GGCGAC-GGCCUUUcGG-----ACUUA-GCGGU- -5' |
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| 18058 | 5' | -55.8 | NC_004680.1 | + | 17426 | 0.69 | 0.497014 |
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Target: 5'- uCCGUUGCCGGAGugGGCUcaUGcggUGCCGu -3' miRNA: 3'- -GGCGACGGCCUU--UCGG--ACuuaGCGGU- -5' |
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| 18058 | 5' | -55.8 | NC_004680.1 | + | 33543 | 0.7 | 0.446975 |
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Target: 5'- aCGuCUGCCGGGAAGCCgu--UCGUUu -3' miRNA: 3'- gGC-GACGGCCUUUCGGacuuAGCGGu -5' |
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| 18058 | 5' | -55.8 | NC_004680.1 | + | 50491 | 0.72 | 0.364536 |
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Target: 5'- gCCGCggGCCGGAAGGUC-GAccucAUCGCUu -3' miRNA: 3'- -GGCGa-CGGCCUUUCGGaCU----UAGCGGu -5' |
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| 18058 | 5' | -55.8 | NC_004680.1 | + | 11074 | 0.73 | 0.323501 |
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Target: 5'- cCUGCUGCCGGgcGGCCUuGGUgGCg- -3' miRNA: 3'- -GGCGACGGCCuuUCGGAcUUAgCGgu -5' |
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| 18058 | 5' | -55.8 | NC_004680.1 | + | 28383 | 0.78 | 0.156446 |
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Target: 5'- gUGCUGCUGGguAGCCgaUGggUUGCCAc -3' miRNA: 3'- gGCGACGGCCuuUCGG--ACuuAGCGGU- -5' |
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| 18058 | 5' | -55.8 | NC_004680.1 | + | 12925 | 0.85 | 0.04769 |
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Target: 5'- gCGCUGCUGGGAAGCCUGGccaGCCAg -3' miRNA: 3'- gGCGACGGCCUUUCGGACUuagCGGU- -5' |
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| 18058 | 5' | -55.8 | NC_004680.1 | + | 3063 | 1.1 | 0.000787 |
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Target: 5'- cCCGCUGCCGGAAAGCCUGAAUCGCCAu -3' miRNA: 3'- -GGCGACGGCCUUUCGGACUUAGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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