Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 18058 | 5' | -55.8 | NC_004680.1 | + | 33113 | 0.67 | 0.657999 |
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Target: 5'- gCCGCgacGUCGaaGAGGGCCgc-AUCGCCAc -3' miRNA: 3'- -GGCGa--CGGC--CUUUCGGacuUAGCGGU- -5' |
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| 18058 | 5' | -55.8 | NC_004680.1 | + | 33543 | 0.7 | 0.446975 |
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Target: 5'- aCGuCUGCCGGGAAGCCgu--UCGUUu -3' miRNA: 3'- gGC-GACGGCCUUUCGGacuuAGCGGu -5' |
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| 18058 | 5' | -55.8 | NC_004680.1 | + | 36518 | 0.66 | 0.726809 |
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Target: 5'- uCCGCuUGCCGGGucuugaccgcaaaguAGGUCUG---CGCCGc -3' miRNA: 3'- -GGCG-ACGGCCU---------------UUCGGACuuaGCGGU- -5' |
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| 18058 | 5' | -55.8 | NC_004680.1 | + | 44149 | 0.66 | 0.679737 |
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Target: 5'- gCUGCUGCugCGGAggugaccgaauGAGCCUGAGUUucaaaCCAg -3' miRNA: 3'- -GGCGACG--GCCU-----------UUCGGACUUAGc----GGU- -5' |
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| 18058 | 5' | -55.8 | NC_004680.1 | + | 44308 | 0.66 | 0.72259 |
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Target: 5'- cCCGCUGCCGcuAGGCCcGGAcagUGCg- -3' miRNA: 3'- -GGCGACGGCcuUUCGGaCUUa--GCGgu -5' |
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| 18058 | 5' | -55.8 | NC_004680.1 | + | 45277 | 0.67 | 0.668885 |
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Target: 5'- uUCGCUGaCGGguGGCUUGu-UCGUCAa -3' miRNA: 3'- -GGCGACgGCCuuUCGGACuuAGCGGU- -5' |
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| 18058 | 5' | -55.8 | NC_004680.1 | + | 47850 | 0.67 | 0.636167 |
|
Target: 5'- -gGCUGCggCGGuuGGCC-GggUCGCUg -3' miRNA: 3'- ggCGACG--GCCuuUCGGaCuuAGCGGu -5' |
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| 18058 | 5' | -55.8 | NC_004680.1 | + | 50491 | 0.72 | 0.364536 |
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Target: 5'- gCCGCggGCCGGAAGGUC-GAccucAUCGCUu -3' miRNA: 3'- -GGCGa-CGGCCUUUCGGaCU----UAGCGGu -5' |
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| 18058 | 5' | -55.8 | NC_004680.1 | + | 57702 | 0.67 | 0.657999 |
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Target: 5'- aUCGUgucCCGcGAAAGCCUGAaccucAUCgGCCAa -3' miRNA: 3'- -GGCGac-GGC-CUUUCGGACU-----UAG-CGGU- -5' |
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| 18058 | 5' | -55.8 | NC_004680.1 | + | 58747 | 0.66 | 0.679737 |
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Target: 5'- -aGCUacggGuuGGGGAGCCgcGGUCGCCGg -3' miRNA: 3'- ggCGA----CggCCUUUCGGacUUAGCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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