Results 1 - 20 of 38 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
18060 | 3' | -51.2 | NC_004680.1 | + | 20156 | 0.66 | 0.933539 |
Target: 5'- uGAuuUGUuguCGCCGGAGgaUCA-GGCGGCg -3' miRNA: 3'- gCU--ACAu--GUGGCUUC--AGUaCCGUCGg -5' |
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18060 | 3' | -51.2 | NC_004680.1 | + | 25429 | 0.66 | 0.933539 |
Target: 5'- cCGAgcuugACGCCGAAc---UGGCGGCCc -3' miRNA: 3'- -GCUaca--UGUGGCUUcaguACCGUCGG- -5' |
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18060 | 3' | -51.2 | NC_004680.1 | + | 34560 | 0.66 | 0.933539 |
Target: 5'- ----cUGCGCCGcccauGUUGUGGguGCCg -3' miRNA: 3'- gcuacAUGUGGCuu---CAGUACCguCGG- -5' |
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18060 | 3' | -51.2 | NC_004680.1 | + | 19631 | 0.66 | 0.927895 |
Target: 5'- uCGAaaccUGUugGCCGAGGcCcgGGCAcGUUc -3' miRNA: 3'- -GCU----ACAugUGGCUUCaGuaCCGU-CGG- -5' |
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18060 | 3' | -51.2 | NC_004680.1 | + | 44868 | 0.66 | 0.927895 |
Target: 5'- aCGGUGgcggagUACCGAAaaCAagcgcUGGCGGCCg -3' miRNA: 3'- -GCUACau----GUGGCUUcaGU-----ACCGUCGG- -5' |
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18060 | 3' | -51.2 | NC_004680.1 | + | 24966 | 0.66 | 0.927895 |
Target: 5'- gCGAUGUG-ACCGGAG-CAagcggUGG-AGCCg -3' miRNA: 3'- -GCUACAUgUGGCUUCaGU-----ACCgUCGG- -5' |
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18060 | 3' | -51.2 | NC_004680.1 | + | 47910 | 0.66 | 0.927895 |
Target: 5'- gGAUccgACACCGGAGcCGcggguguuUGuGCGGCCg -3' miRNA: 3'- gCUAca-UGUGGCUUCaGU--------AC-CGUCGG- -5' |
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18060 | 3' | -51.2 | NC_004680.1 | + | 26024 | 0.66 | 0.921962 |
Target: 5'- uCGAUGguCACCacGGcaUCAUGGCGGCg -3' miRNA: 3'- -GCUACauGUGGcuUC--AGUACCGUCGg -5' |
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18060 | 3' | -51.2 | NC_004680.1 | + | 1239 | 0.66 | 0.921962 |
Target: 5'- aCGuUGUACACguCGAcGgcugGGCAGCCg -3' miRNA: 3'- -GCuACAUGUG--GCUuCaguaCCGUCGG- -5' |
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18060 | 3' | -51.2 | NC_004680.1 | + | 27824 | 0.66 | 0.921962 |
Target: 5'- gGAUGgUugGCCGcAGcUC--GGCGGCCg -3' miRNA: 3'- gCUAC-AugUGGCuUC-AGuaCCGUCGG- -5' |
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18060 | 3' | -51.2 | NC_004680.1 | + | 7667 | 0.66 | 0.921962 |
Target: 5'- ---cGUuCAUCGAg--CGUGGCGGCCu -3' miRNA: 3'- gcuaCAuGUGGCUucaGUACCGUCGG- -5' |
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18060 | 3' | -51.2 | NC_004680.1 | + | 22198 | 0.66 | 0.915743 |
Target: 5'- uCGAggccGCGCCGAAGUU-UGuGCGGUCg -3' miRNA: 3'- -GCUaca-UGUGGCUUCAGuAC-CGUCGG- -5' |
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18060 | 3' | -51.2 | NC_004680.1 | + | 3713 | 0.66 | 0.909237 |
Target: 5'- uCGGUG-GCACCGGuuGUCcaaGCGGCCc -3' miRNA: 3'- -GCUACaUGUGGCUu-CAGuacCGUCGG- -5' |
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18060 | 3' | -51.2 | NC_004680.1 | + | 23784 | 0.66 | 0.909237 |
Target: 5'- cCGAUGU-CACCGAuGUgAcGGCgAGCg -3' miRNA: 3'- -GCUACAuGUGGCUuCAgUaCCG-UCGg -5' |
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18060 | 3' | -51.2 | NC_004680.1 | + | 11246 | 0.66 | 0.902447 |
Target: 5'- aCGAcGUugcCGCCGggGauUCcUGGCAuGCCg -3' miRNA: 3'- -GCUaCAu--GUGGCuuC--AGuACCGU-CGG- -5' |
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18060 | 3' | -51.2 | NC_004680.1 | + | 42291 | 0.67 | 0.895376 |
Target: 5'- ----cUGCACgGugcGGUgGUGGCAGCCc -3' miRNA: 3'- gcuacAUGUGgCu--UCAgUACCGUCGG- -5' |
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18060 | 3' | -51.2 | NC_004680.1 | + | 5319 | 0.67 | 0.89247 |
Target: 5'- cCGGUGUcggaaaucgcgucCGCCGAGGUC-UGcCGGCCa -3' miRNA: 3'- -GCUACAu------------GUGGCUUCAGuACcGUCGG- -5' |
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18060 | 3' | -51.2 | NC_004680.1 | + | 28563 | 0.67 | 0.888029 |
Target: 5'- -cGUGUGgGCCGA--UCAcGGCGGCUc -3' miRNA: 3'- gcUACAUgUGGCUucAGUaCCGUCGG- -5' |
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18060 | 3' | -51.2 | NC_004680.1 | + | 45842 | 0.67 | 0.888029 |
Target: 5'- uGAUGUACACgguuuCGggGaCGUGGCc-CCa -3' miRNA: 3'- gCUACAUGUG-----GCuuCaGUACCGucGG- -5' |
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18060 | 3' | -51.2 | NC_004680.1 | + | 20892 | 0.67 | 0.888029 |
Target: 5'- --cUGU-CACCGGAGUCGuaugaggcgUGGCuGUCa -3' miRNA: 3'- gcuACAuGUGGCUUCAGU---------ACCGuCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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